Abstract:Five compounds were isolated from the root of Astragalus floridus Benth collected from Qinghai province, they were identified with spectral analysis and chemical methods as astrasiversianin Ⅴ (1), astragaloside Ⅳ (2), astragaloside Ⅱ (3), astrasiversianin VI (4), astramembrannin Ⅱ (5), Pharmacological test showed that astragaloside Ⅳ cound improve heart failure significantly.