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Phylogenetic utility of LEAFY gene in Cinnamomum (Lauraceae): Gene duplication and polymerase chain reaction-mediated recombination


Low copy nuclear genes show great potential to provide phylogenetic information, but their use has been hampered by several inherent adverse factors. Polymerase chain reaction (PCR)-mediated recombination ranks among these factors and occurs when high levels of similar paralogs for a low-copy nuclear gene coexist within a single PCR amplification reaction. In this study, the