全 文 ：Chinese Bulletin of Life Sciences
17 2
2005 4
Vol. 17, No.2
Apr, 2005
100850
2-DE MS 2-DE 2-D DIGE 2-DE
2-DE ICAT
2-DE
ICAT
Q 5 1 A
Advance in differential proteomics research
SUN Yan-Wei, JIANG Ying, HE Fu-Chu*
(Institute of Radiation Medicine, Academy of Military Medical Sciences , Beijing 100850 , China)
Abstract: Differential proteomics is a major research area in proteomics era, which mainly aims to detect and
confirm the different proteins caused by special stimulations between two or more group of samples. By
analysis of the differentiae between samples, in reverse, we can deduce what induce the variety patterns of
expression, hence it is useful in cancer pre-diagnosis, new drug development and cell regulation research. For
differential proteomics research, samples should be carefully dealt with in order to get an accurate and repro-
ducible result, therefore laser capture microdissection and some removing high-rebundant proteins techniques
are used. Now the association of 2-DE separation and MS identification are primarily used in the field, but some
new methods are appearing, for example, 2-DE DIGE, which overcome the demerit of it ancestor and suit this
kind of experiments much better, those techniques other than 2-DE such as multidimension chromatogram,
ICAT and protein chips have also been used, which can complement or substitute the conventional methods.
Key words: differential proteomics; 2-DE; chromatogram; ICAT; protein chips
1004-0374(2005)02-0137-04
2004-11-12 2004-12-16
2002-BA711A11 H030230280390
( 1 9 7 9 ) ( 1 9 7 5 ) 1 9 6 2
*
,
138
,
[1~2]
1
(
)
[3~4]
(2-DE)
(1) ; (2)
; (3)
[5]
(ICAT)
(MS/MS)
2
2.1
(LCM)
Shekouh [6] LCM
Wang [7]
Ahmed [8]
Affi-Gel Blue Aurum
Hynes
[9]
Laukel [10]
Turck [11]
2.2 2-DE
2-
DE MS
pH IPG
E1 Fakhry [12]
pH 3~10 4~7 5~6 6~11
Ahmed [8] pH4~7
2-DE
(2-D DIGE)
Tonge Zhou Yan
DIGE
[13~15]
2.3
2.3.1
ICAT
Hamler [16]
- -
(NPS-RP-HPLC)
pH
-
(ESI-TOF MS)
[ 16]
Allet [17]
2.3.2 ICAT ICAT
ICAT
ICAT
ICAT 8D 4D( )
MS/MS
ICAT
Brand [18]
NF-E2p18/MafK
ICAT
2.3.3
(SELDI-TOF MS)
Petricoin [19]
5 95%
Lal [20]
CD
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