全 文 ：Chinese Bulletin of Life Sciences
17 2
2005 4
Vol. 17, No. 2
Apr., 2005
510515
;
Q503;Q51 A
Protein microarray and proteomics
FEI Jia, MA Wen-Li*, ZHENG Wen-Ling
(Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China)
Abstract: Protein microarray technology has become a crucial tool for large-scale and high-throughput biology.
It allows fast, easy and parallel detection of thousands of addressable elements in a single experiment. This
article reviewed protein chips great potential in proteomics research. It has been applied to isolate, quantify
protein and analyse protein-protein, protein-small molecule interaction. Protein chip combinated with mass
spectrometry, biosensor technology expended its applicable scale. It also introduced the advance of proteome
chip and alive chip in proteomics research. Protein microarray plays an important role in proteomics research.
Key words: protein microarray; proteomics
1004-0374(2005)02-0132-05
2004-06-03 2004-07-14
39880032
( 1 9 6 4 ) ( 1 9 6 4 ) *
( 1 9 6 2 )
[1]
1
(two dimensional gel electrophoresis,
2DGE) (mass spectrometry, MS)
1.1 1975
[2]
pH (Isoelectric focusing);
SDS-PAGE
20 80 Bjellqvist [3]
pH pH
2DGE
2DGE
1.2
(bio-mass spectrometry)
(m/z)
20 80
(electrospray ionization ESI)
(matrix-assisted laser desorption ionization MALDI )[4~5]
2
Kodadek[6]
( )
(1 )
(2 )
(3) ELISA
3
3.1 2DGE
[7]
D N A
(1)
( ); (2)
-
von Eggeling [8]
3.2 Mendoza
[9] ELISA
96
96
36 8
96
134
13 824
ELISA
C C D ( c h a r g e
coupled device) AIDA
Bacarese-Hamilton [10]
5
IgG IgM
0.5pg ELISA 80%
Copeland [11]
ELISA
3.3
[12] Gray
[13]
(Cdc28p)
Cdc28p
3.4 (Lab-on-chip) DNA
[14] Chiem [15]
7.6cm .6cm
( 1 )
( 2 )
( 3 )
W o l f [ 1 6 ]
(microfluidic networks, microFNs) C
(CRP)
10 1
CRP 30 ng/ml
C R P
3.5 -
(surface enhanced laser desorption/ionization-
time of flight-mass spectrometry, SELDI-TOF-MS)
[17]
- -
- -DNA
(1)
(2) (
); (3)
( Western
)[18] Davies
[19] -
β (amyloid-β peptide A β)
-Aβ( )
-Aβ
Aβ
SELDI-TOF-MS
Aβ
Koopmann [20] SELDI
60
60 60
78% 97%
CA19-9(P<0.05)
CA19-9
3.6
Pharmacia Biotech
(biomolecular inter-
action analysis, BIA)
(surface plasmon resonance,SPR)
SPR
BIA
MALDI-TOF-MS
[ 2 1 ]
3.7

5 800
5 800
Zhu [22]
5 800
DNA
4
Zhu [22]
(proteome chip)
3.8
( )
Uetz [23]
6 000
(opening read frame
ORF) Gal-4
D N A -
RNA- 192
281 -
[24]
4
136
,
(2DGE) (MS)
/
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