免费文献传递   相关文献

Establishment and evaluation of predictive model for growth of  Aeromonas spp. from chilled pork

冷却猪肉中气单胞菌生长预测模型的建立和检验



全 文 :!10
"!

#
2012
$

%
/
 
X
 
Ð
 
O
 
²
 
³
ChineseJournalofBioprocessEngineering
Vol.10No.2
Mar.2012
doi:10.3969/j.issn.1672-3678.2012.02.011
NOPH
:2010-11-26
JQR!

ÜÝn˜ÔpÍØÙÚÛ
(30800864)
ST.U

IÝö
(1979—),
 

£Ò;tí



x§¨

89ñò

E0{lû ‚u!g
,Email:dongqingli@126.com
²³‹´e8f$t[_µ§ë‚¯¶qœµ
päå
1,
æ
 
ç
1,
è
 
é
2,
êëë

(1
ÜÝmXW1 âã018XYFGH

ÜÝ
200093;
2
Ò//ìUíW1 âã0185>[\10

Ò/îï
200701)
7
 
a

µWXYZ?[<Í÷’˪·_\]
,^
Í÷’Ë¿Zãw
80℃
_Ëí`˪YZ?[<
,¯
u
Gompertz
­
Baranyi
6aêÁb¥cÍ÷’Ë8§Ià©:ª·_de

ÇÈÚs

fTª
Gompertz
êÁÌÍ÷
’Ë·_ghªëó³Èû„
Baranyi
êÁ
,R2
8
097
¦|

B1qriêÁ­
Arhenius
êÁ̽fTª
Gompertz
êÁ¶ªª+jæ·_UÔÀÁëó

W¶qriêÁëó³Èkû„
Arhenius
êÁ

RSWu¼aê
ÁþS
0~35℃
lmØÙ:Í÷’˪·_

bcd

Í÷’Ë

YZ?[

êÁ

qri
;Arhenius
efghi
:TS2013,S4313    
6jklm
:A    
6n;i
:1672-3678(2012)02-0050-05
Establishmentandevaluationofpredictivemodelforgrowthof
Aeromonassppfromchiledpork
DONGQingli1,GAOCui1,DINGTian2,LIYuanyuan1
(1InstituteofFoodScienceandEngineering,UniversityofShanghaiforScienceandTechnology,Shanghai200093,China;
2DepartmentofFoodScienceandBiotechnology,KangwonNationalUniversity,Chuncheon200701,Korea)
Abstract:ToverifythegrowthruleofAeromonasspp.fromthepork,theporkwasinoculatedwith
Aeromonasspp.afterdisinfectedby80℃ sterilewater,andprimarymodels(GompertzandBara
nyi)weredevelopedtofindthebestfitingmodeltodescribethegrowthkineticofAeromonasspp..
Byacomparison,thefitingefectofmodifiedGompertzmodelongrowthcurveofAeromonasspp.
wasbeterthanthatofBaranyimodel,withalthevalueofR2beyond097.Themaximumspecific
growthrategainedfrommodifiedGompertzmodelwasfitbysquarerootmodelandArrheniusmodel,
respectively,andthefitingefectofsquarerootmodelwasbeterthanthatofArrhenius.Theresults
indicatedthatthesecondarymodelwasausefultoolforpredictinggrowthofAeromonasspp.in
chiledporkfrom0-35℃.
Keywords:Aeromonasspp.;chiledpork;model;squareroot;Arhenius
  
š‘›K$ŒJu7bÄv7vvãä~³K

7Teî$OmIqíEþï7ï•K

$I¨îw
x@w‰ä"7îïK
[1-5],
Qçï$—˜‰™V7
Q0†‰K
[6]。
ÜUi…º$A—˜‰™Væx
³K

y‘›KPz7:ã7ï•KÎG89

k6
š‘›K%&89A27xÃ?z

  
Ãó7W/X –ñËW;²³u

kásM
MÞïE˯3G–w!g7Û7
[7]。
q`W/
Xœ–c7ñËI6AW/X7/uw¯Ž%
&ÎGœx

Ikœ–™{7{ô#wlûv

„
…Sš‘›Kwxxm3—˜‰™V

ˆš‘›
Kk
0~35℃
=7/u%&

žŸ/uœ–c

6
#&žŸ—˜‰™Vš‘›K7DI|Ԕ>
¹p‹

1 
op%rs
11 
op
  
}ô±…Q‚Â~™

†J±Æ€èn:
“

æJ
0~4℃
¢£V—‡h`

12 
tu‚覑k¨
  
š‘›K
(No23564)
"¡JVÜO€W/X
Km‡¤>VÆ

‡Jš‘›K/“š›p
V
,27℃
•F

:

gMÎÏKÐ/

š›p7Á
gañŠ¯
[8]。
13 
š·¸uq¹º
  
ÿ25g
™‹ŠJ
80℃
EKDV
10s
ÎG
žK

S±MÎÏKÐ/`EK/>D
(083%
NaCl
Ÿ/

ÎGO”7U]¯°*

SžK*™‹
ŠJjV
15s
ÎGxm

ÎÏxmu³&
102~
104
`
/g。
Sxm*™‹âJEKV

~†æ
J
0、4、10、15、25、30
w
35℃
©ªš›£V‚‡

14 
ú[ŠðM
  
«]À®7ç)\@O”u7â>
25g
™7
EK
,`
ƒñS™­ƒÝ*æJâ>
225mL
žK
/>D7èéçV

û~FGµ
,¨ 10
­(‚
¯°3sF¯°]

añŠ¯
[9]
7ñË`B>Ö
œË–”•Kœ

jVš›pañŠ¯
[10]
ƒÖ
7š‘›K`J㆚›p

15 
»¼§ë‚½“
  
Oüc7Ygò`ÄÜ
OriginLab
ÇÈ7
Or
igin80
’y

  
jû7
Gompertz
c߂
(1)[11]:
Nt=A+Cexp{-exp[-B(t-M)]} (1)
‚V
:t
&ç)
,h;Nt& tçKœ7Ó`Aœª;A、
B、C、M
$caœ

ojû7
Gompertz
ñ³Yg
M@

  
LM

+æñ/uÒ¶
U(h-1)、
w„#ç)
tlag(h)w+æʛz] Dm,Io‚(2)~‚(4)Ö
ÂM@

U=BCe (2)
jV
e=27182。
tlag=M-

B (3)
Dm =A+C (4)
  Baranyi
c߂
(5)~
‚
(7)。
+ÑK@œ

IoÎÏK@œ
Y0、w„#K@œ Y1w/u#K

Y2ÖÂM3。
Y=Y0+
Y1
ln10+
Y2
ln10 (5)
Y1 =μmt+ln[e
-μmt-e-μm(t+tlag)+e-μmtlag](6)
Y2 =ln[1+10
(y0-ymax)(eμm(t-tlag)-e-μmtlag)](7)
jV
:y0w ymax~†$w„#+ÎK@œw+ÑK

;μm$+æñ/uÒ¶(U)。¸²…. y0、ymax、
μm tlagÎGaœ|Ö,6lmÇÃBñw(RS):
RS =∑

i=1
[y(ti,y0,ymax,μm,tlag)-yi]
2 (8)
16 
X¼§ë‚½“
  
ò`
Origin80
’yAî¡sMœ¼ÎGÁv
ˆS

¬`Bñ»cw
Arhenius
cAª] /
uÒ¶ÀÁÎGYg
,`
689ª]AJOüc
Vaœ7GH

  
Bñ»c߂
(9)。
槡U =αμ(T-Tmin) (9)
‚V
:a
μ&/uÒ¶ÁvˆS7J¶
;Tmin$O`y
ƒ7†‡

>¹±$íW/X/u>.^•rç
7ª]
(℃)。
6±c7À`9:$k
[Tmin,
Topt],jVTopt$W/X/u7+˻]
[12]。
17 
§ë‚œµ
  
q`ˆ”úœ
(R2)(¸
²Yg’yM@
)、
ò!

(BF)w¿ÔLÂ(AF)I”c,ǂú=:
BF =10
∑lg(Upred/Uobs)
n (10)
AF =10
∑|lg(Upred/Uobs)|
n (11)
jV
,Upredw Uobs~†&+æñ/uÒ¶7œ–ª
w

2 
yz%{)
21 
8f$t»¼§ë‚2¯
  
¬`jû7
Gompertz
c
、Baranyi
cYg
0~
35℃
=—˜‰™Vš‘›K7/uÀÁ

sMˆ”
úœúƒ

s8





jû7
Gompertz
c
15 
!

# IÝöP

—˜‰™Vš‘›K/uœ–c7žŸw ¡
sM
R2
ªk
097


?
Baranyi
c#x!
10,
6=89¹$
Gompertz
cÞ+üc

3

&
0~35℃
=q`jû7
Gompertz

˜‰™Vš‘›K/u7YgÀÁ

o3



ª]Aš‘›K7/uGHé/

®š›ª]&

w
4℃
ç

š‘›K/u?&0?

k,
50h
U

š
‘›KœÛpK*ÐW.

®š›ª]WXÍà
ç

š‘›K7/uïâ)Ð=

]†$ª]¯3
30
w
35℃
ç

!N
30h,
š‘›K/uN¯3[²
”#

…
1 Gompertz
q
Baranyi
§ëP”•ŽŒ¹‚¾ß¿MÀ
(R2)
Table1 R2valuesobtainedfromGompertzandBaranyimodelsatdiferenttemperatures
c
R2
0℃ 4℃ 10℃ 15℃ 20℃ 25℃ 30℃ 35℃
Gompertz
c
09799 09954 09948 09831 09916 09912 09897 09736
Baranyi
c
09665 09419 09522 09455 08136 09355 09359 08925
f
1 
²³‹´P”•¹ºŽŒe8f$t‚[_°±
Fig.1 GrowthcurvesofAeromonassppinchiledpork
atdiferenttemperatures
  
jû7
Gompertz
c7aœYgMÞúƒ

s8





®š›ª]Íàç

š‘›K7
+æñ/uÒ¶

ïâ)Íà

k/uw„#
tlag}
~

®ª]I
0℃
ꈦ
10℃
ç

+æñ/uÒ¶
o
0028h-1
Íà3
0128h-1。
LM

!g—‡ª
]mJ
4℃,
>öJâ-™ó†‰

wu{ô#1[
àlûv

22 
8f$tX¼§ë‚2¯
  
~†q`Bñ»c

3
2)
w
Arhenius
c

3
3)
"•Mª] š‘›K]”reaœ))7¼
ú

o3

w3



Bñ»c7
R2
&
09678,
   
…
2 
”•ŽŒ¹8f$t
Gompertz
§ë‚[_LM
Table2 GompertzmodelparametersofAeromonassppatdiferenttemperatures
‚‡ª]
/℃ A B C M U tlag Dm
0 2179±0342 0012±0004 6549±1087 1531±15200 0028 66140 8727
4 3376±0708 0021±0002 4279±0144 9981±3108 0033 52190 7655
10 2417±0151 0055±0006 6354±0286 3072±1413 0128 12500 8771
15 3083±0242 0056±0010 4183±0350 2719±2558 0087 9471 7266
20 1782±0189 0082±0013 6106±0282 1958±0836 0185 7425 7889
25 2535±0214 0130±0023 5296±0337 1315±1004 0253 5458 7832
30 2117±0217 0152±0020 5510±0264 1190±0767 0309 5329 7630
35 2110±0366 0176±0036 5310±0461 9459±1144 0345 3792 7410
 
T

jV
A、B、C、M
~†&
Gompertz
caœ
;U
&+æñ/uÒ¶
(h-1);tlag&w„#ç)(h);Dm&+æʛz]。
7àJ
Arhenius
c7
09659,
7…!†Wæ

Mgñ!~–MÞ"º

¹$Bñ»c&š‘›
Kñ+æ/uÒ¶7+üœ–c

Yg7+ü
c7œ–ªpK1@kœ–ÀÁ7
95%
æð
˜)U

LMc7œ–MÞ$Ið7

sž+ü
cú=

槡U =00133(T+10901)
  
ê NŒ‘
[6]
k—˜‰™VW/X/~–
25
/
 
X
 
Ð
 
O
 
²
 
³
  
!
10
"
 
i{ô#œ–c789VsM7MÞ½F

6f
c•M7ª] +æñ/uÒ¶7¼ú$I
ð7

f
2 
8f$t‚3Á–[_©%ŽŒÄ‚Ãrħë
Fig.2 Squarerootmodeloftemperature
dependenceofU
f
3 
8f$t‚3Á–[_©%
ŽŒÄ‚
Arrhenius
§ë
Fig.3 Arrheniusmodeloftemperature
dependenceofU
23 
§ë‚œµ
  
`sM7+ücAœ–ªwÎG½
¼v~–

MÞú3

s8

o3


R2
&
09662,
6fœ–ªw7½¼v?à

f
4 
8f$tµÀqÀÀ‚ób`gx
Fig.4 Relativeanalysisofobservedversuspredicted
growthratevaluesofAeromonasspp
  
Qçï-]

®xmu?nç

úmJ
10-1
`
/g
ç
,25℃
=š›`jû7
Gompertz
cAMÞÎG
YgsMÀÁú3

s8

sM
R2=09949,

cYgµÞ÷>¯

;$îÿ–M7+æñ/u

Uobs=03310,k+ücM325℃š›xy=
7œ–ª
Upred=02280,MÞ½!÷æ。s6ÎÏ
KuAc7À`9:ïŽk÷æGH

k6*
789Vïq+6ª5

f
5 
´³¸u€—H+²³‹´e
8f$tP
25℃
¹‚[_°±
Fig.5 GrowthcurvesofAeromonassppinChiled
porkat25℃ withsmalerinitialinoculation
quantity
  
f”úœ
(R2)、
ò!LÂ
(BF)w ¿ÔLÂ
(AF)` "%& ¡c7œaœ,jV BFw AF
T®&$¡ec7(Ó>øª7O_
[13]。
ò!
LÂw¿ÔL¹x!J

ƒ8c7œ–µÞ¹
D
。Ross
®&AJW/X/uc7¡e

úÞ
BF
ªk
090~105
79:)U

cp6÷Dõœ
–W/X/uÒ]w/uÛ&

úÞ
BFªk070~
090

106~115
9:)U

½c$I6
Tx27

úÞ
BFªæJ115<…nJ070,½
6fc$Ž‰7

K89sM7
BFª&
09615,
k
090~105
))

6fÞ7cp6
÷Dõœ–W/X7/uÛ&i/uÒ¶

®
AF=1ç,6fc>÷à7ÂÔv,œª¹æ<…
¹n

6fc7¿Ôv¹m
[14]。
Kî¡sM
AF=10401,(Óx!1,s6ê`MÞñ?>¯,
6fcp?Dõœ–î¡Œš›xy=
0~
35℃
U—˜‰™Vš‘›K7+æñ/uÒ¶

3 
y
 
)
  
žŸ[
Gompertz
w
Baranyi2
mÎüc
,`
6
•M—˜‰™Vš‘›KkWQª]=7/u%
35 
!

# IÝöP

—˜‰™Vš‘›K/uœ–c7žŸw ¡
&。
AñMރf
,Gompertz
c7
R2
k
097


p6#DõYg—˜™Vš‘›KkZ`ª
]=7/uÛ&

ñ?Bñ»c
Arhenius
c
Aojû7
Gompertz
cM@7+æñ/uÒ¶
ÎGYgµÞ

sMBñ»cYgµÞ7QJ
Arhenius
c

AcÎG7œ ¡Mރf

M+ücI6÷Dõœ–š‘›Kk½qIB
=7/u%&

L˜6j

[1] 
mdc

i_‰
560
-ÉvEŠïþ…š‘›Kċ‹ý
[J].
VØGï,%
,1997,18(6):153155.
[2] 
NŒ)

ËøÕ

ȍÆ

P

ë—4“ŽDîïv
Dš‘›K7îý
[J].
Vܑb,%
,2002,38(6):4546.
[3] 
’*}

[‘å

OÆoDš‘›KÅÆ7…XVÛîý
x4
[J].
VÜï•/X,%
,2010,5(8):2.
[4] 
Ît

÷Ãö

“B7

š‘›KŠ‹wx³õ…{7îý~
–
[J].
].œâb
,2001,28(4):526527.
[5] 
Àu

ulÜ

˔K

P

š‘›KWQm7Giqìv
[J].
V܌ċFõ,%
,2004,4(5):302305.
[6] 
NŒ‘

—˜‰™VW/X/~–i{ô#œ–c78
9
[D].
–—

–—€æ
,2006.
[7] McdonaldK,SunDW.Predictivefoodmicrobiologyforthemeat
industry:areview[J].IntJFoodMicrobiol,1999,52:127.
[8] 
÷Ûâ

W/Xš›p7g± q`
[M].
ë—

VÜ€@
™b
,1995:250579.
[9] 
VØí¿¥wܘ/8
.GB/4789.2—2008
…{˜/W/
X ¡K@圖”
[S].
ë—

Vܾ¿@™b
,2009.
[10] MccoyRH,PilcherKS.Peptonebeefextractglycogenagar,ase
lectiveanddiferentialAeromonasmedium[J].JFishResBoard
Canada,1974,31(9):15531555.
[11] GibsonAM,BratchelN,RobertsTA.Theefectofsodiumchlo
rideandtemperatureontherateandextendofgrowthofClostridi
umbotulinumtypeAinpasteurizedpokslury[J].JApplBacteri
ol,1987,62:479490.
[12] 
o«c

Ëd

È«

P

—˜‰™Vy‘›K/uœ–c
7žŸ ¡e
[J].
ꖀԁ
,2010(1):128131;134.
[13] TeGifelMC,ZwieteringMH.Validationofpredictivemodels
describingthegrowthofListeriamonocytogenes[J].IntJFood
Microbiol,1999,46:135149.
[14] 
3a

IÝö

½C•

P
.Listeriamonocytogenes
kìàäF
«•Y@vD=>7…–—±œ–c7žŸ
[J].
…
{O€ÔÕ
,2010,31(3):8790;
櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒櫒
94.
,-./
ÅÆÇÈÉ4=z
:RNA
%gZÊË̕S¦4§Æ
VÜÔÕÕáæ/¼Ôâ7˜™â¥kå89V08[æx³K
Hfq
vr“k n
RNA
DsrA
Mg²³VnQ%`7坂

½¼89¹Š-ƒk
《Genes&Development》
±

kꚹŠV

89
íðE@[
Hfq
 
DsrA
±O(
RNA
¨(

ê$O(
Hfq
 
DsrA
Mg˜$
)AU6A(AUUUUUUA)、
 
ADP
èÛ]gX7N“MÃ6i
Hfq
 
ADP
+Û]gX7N“MÃ

-]
Hfq
 
DsrA
Mg˜$Mgi 
ADP
~ÂMgFN
2` Hfq


~†¬`
2`
WQ7~ƒ„nQ%`

N“MÃé8
:AU6A
 
Hfq
7Mg
¬\[WQJ7ã7
RNA
 
Hfq
7Mg‚

)@¥FFY÷±rî¡w˜@›œ‚ƒî¡6fkŸ
/V
Hfq
 
DsrA
7Mg6i 
ADP
~ÂMgï¬\½Q‚

pº¥Fpud÷fûu7
DsrA
 ûu7
Hfq
7MgÔî×i

`
Hfq


±MO%«-][,íëã7 
ADP
Mg7¼.Íp@
¯p


{Am

45
/
 
X
 
Ð
 
O
 
²
 
³
  
!
10
"