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Porcine pancreatic lipase immobilised on nano-silver mesoporous activated carbon composite

纳米银复合介孔碳材料制备固定化猪胰脂肪酶



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ChineseJournalofBioprocessEngineering
Vol.10No.3
May2012
doi:10.3969/j.issn.1672-3678.2012.03.010
OPQI
:2011-08-02
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:1672-3678(2012)03-0050-06
Porcinepancreaticlipaseimmobilisedonnanosilvermesoporous
activatedcarboncomposite
YANGBo,WANGWei,ZHUKai,WANGHuan,HANPingfang
(ColegeofBiotechnologyandPharmaceuticalEngineering,NanjingUniversityofTechnology,Nanjing210009,China)
Abstract:Thestudywascariedouttoimmobilizeporcinepancreaticlipase(PPL)onnanosilvermeso
porousactivatedcarbon(AgMAC).MACwaspreparedfromricehuskbyatwostageprocessandnano
silverwasabsorbedonthesurface.PPLwasthenimmobilizedonAgMACandconditionswereopti
mized.Resultsshowedthatundertheconditionsofthesilvernitrateconcentrationof001mol/L,immo
bilizationtimeof6h,reactivephosphatesolutionofpH6.0,reactiontemperature25℃,thelipaseac
tivityincreasedby3.2times.Meanwhile,N2adsorptiondesorption(BET),scanningelectronmicroscopy
(SEM),Fouriertransforminfraredspectroscopy(FTIR)wereusedtoanalyzeexperimentalresults,and
confirmtheefectsofAgMACinexperimentsoflipaseimmobilization.
Keywords:mesoporousactivatedcarbon;immobilization;nanosilver;porcinepancreaticlipase
  
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