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作 者 ：杨朗,梁广文,曾玲,岑贞陆

期 刊 ：生态学报 2008年 28卷 8期 页码：4015~4022

关键词：水稻白叶枯病；高效液相色谱；次生物质；模型；

Keywords:Xanthomonas oryzae pv. Oryzae, HPLC, secondary compounds, model,

摘 要 ：应用高效液相色谱分析了对白叶枯病具有不同抗性水平的12个水稻品种中的19个(组)次生物质色谱峰（面积）的差异及其与白叶枯病抗性水平间关系。结果表明，水稻品种抗性水平与谱峰面积值之间相关极显著（R=0.992，p<0.01），被测的19个组分中，峰1、峰2、峰8、峰10、峰12、峰14、峰16和峰18是影响水稻对稻白叶枯病抗性水平的主要抗原次生物质。建立了水稻品种对白叶枯病抗性级别与以上次生物质含量谱峰面积之间的回归模型：Y=10.7603+0.1823X1-0.2287X2+02163X8-2.1975X10+ 0.0728X12 -0.7438X14+ 1.1484X16-0.7795X18。研究结果表明水稻品种中起抗病作用的抗原次生物质不止一种，而是几种的组合，而且它们对水稻抗病性的贡献作用是不完全相同的，这与它们的性质与含量密切相关。提出了以抗原次生物质为标记的快速分析、鉴定、预测水稻品种对稻白叶枯病抗性水平的新途径、新方法。

Abstract:The relationship between levels of 19 secondary compounds in rice leaves and resistance of 12 rice varieties resistance to Xanthomonas oryzae pv. oryzae was investigated using high-performance liquid chromatography(HPLC). The relationship between level of X. oryzae resistance amd the area values of chromatographic peaks were significant (R’=0992，p<0.01). A resistant predictive model was established through multiple regression analysis: Y=10.7603 + 01823X1- 0.2287X2 + 0.2163X8 - 2.1975X10 + 0.0728X12 - 0.7438X14 + 1.1484X16 - 0.7795X18. This model indicates that peak 1, peak 2, peak 8, peak 10, peak 12, peak 14, peak 16 and peak 18 are major secondary compounds that may influence varietal resistance to X. oryzae. The resistance of rice varieties to X. oryzae was closely associated with qualitative and quantitative combinations of the secondary compounds, suggesting that X. oryzae resistance arises from the combined action of several secondary compounds that vary in contribution to resistance of the varieties. These secondary compounds may be useful as markers to evaluate and identify the rice varietal resistance to X. oryzae.

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