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作 者 ：杨春平, 宁红, 陈华保, 李钊君, 张敏

期 刊 ：核农学报 2016年 30卷 6期 页码：1157-1162

关键词：4-D；残留量检测；高效液相色谱；固相萃取；

Keywords:2, 4-D, residue detection, high-performance liquid chromatography, solid phase extraction,

摘 要 ：为建立蔬果中2,4-D残留量高效液相色谱(HPLC)检测方法,以固相萃取为净化手段,采用HPLC法检测番茄、葡萄、枇杷和黄瓜4种蔬果中2,4-二氯苯氧乙酸(2,4-D)的残留量。结果表明,样品经碱性水溶液提取、酸化,C₁₈固相萃取小柱净化后,能够有效排除杂峰干扰,2,4-D残留量检测准确。该方法的线性相关系数为 0.9980,检测限为0.0012 mg·kg^-1,2,4-D的添加回收率为81.8%~116.0%,变异系数(CV)小于6%,表明方法准确度高,能满足番茄、葡萄、枇杷和黄瓜产品中2,4-D残留量的检测。采用此方法测定番茄等4种蔬果21份样品,样品中仅有2份番茄样品检测出2,4-D,残留量均小于0.02mg·kg^-1。本研究为利用HPLC检测果蔬中2,4-D残留量奠定了理论基础。

Abstract:In order to establish the determination of 2,4-dichlorophenoxyacetic acid(2,4-D) residues by high-performance liquid chromatography(HPLC),four vegetables and fruits of tomato, grape, loquat and cucumber were determined by solid phase extraction and high-performance liquid chromatography (SPE-HPLC) in this paper. The sample was extracted and acidified with alkali aqueous solution, and purified by the SPE-C₁₈ column. The results indicated that the analysis method was accurate, with correlation coefficient of 0.9980, and detecting limit of 2,4-D acid were 0.0012 mg·kg^-1, and the average recoveries ranged from 81.8% to 116.0%, and the coefficient of variation (CV) was less than 6%, which can be used for determined 2,4-D acid residues in above-mentioned four kinds of vegetables and fruits. 2,4-D acid residues in 21 samples of four kinds of vegetables and fruits were determined in this method, and only 2 tomato samples were determined 2,4-D acid residues, with less than 0.02 mg·kg^-1. The above experiments provided basis for the detection of 2,4-D acid residues in 4 kinds of fruits and vegetables by HPLC.

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