Abstract:In order to establish a rapid method for evaluation the freshness of PortunusTrituberculatus, the changes of volatile components in flesh during storage at 4 ℃were investigated by electronic nose, and the freshness were analyzed through principal component analysis (PCA) and linear discriminant analysis (LDA) coupled with pH, TVB-N and total viable counts. The results showed that the response of electronic nose gradually increased with the extension of storage period. BothPCA and LDA analysis could identify the flesh of PortunusTrituberculatus undergoing different storage periodeffectively. Based on the response of electronic nose and partial least squares regression (PLSR) prediction model related to pH, TVB-N and total viable counts, the (RPD) value were 2.73, 2.50, 3.06, respectively, which suggested that the PLSR model could be utilized for the prediction of changes of pH, TVB-N, total viable counts. The calibration and validation value were both above 0.85, which showed that the response of electronic nose had a good correlation with physicochemical parameters and total viable counts. Therefore, the advancement made in our study will provide techniques and theoretical basisfor the evaluation of the freshness of aquatic product as well as the effect of different preservation method.