Abstract:The exotracelluar polysacchrides (EPS )were isolated and purifled from A. faecalisA1501 and its mutants A1513,A1532,A1513,and A1523.EPS of these bacteria contained sugar and polypeptide and consists of two distinct fractions:EPS component of high molecu-lar weight and low molecular weight.The molecular weight of HMW component was 250kD,and that of LMW component was less than 40kD. Both of them contained glucose, galactose,fructose and some pentose,and the ratio of glucose,galactose for wild type and its mutantswas as follows :A1501 :EPSH 2 :1 , EPSL3:1;A1513EPSH2 : 1 , EPSL1:1; A1532 EPSH8:1 ,EPSL5:1;A1513 EPSH,EPSL 4:1; and A5123EPH 4: 1,EPSL8:1.There was apolypeptide component in the HMW fraction. The amino acid compositions were differentamong tbe tested strains.