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作 者 ：魏源文，胡春锦，邓智年，李杨瑞

期 刊 ：热带亚热带植物学报 2007年 15卷 4期 页码：290~293

关键词：甘蔗；乙烯利；cDNA-AFLP；基因差异表达；

Keywords:Sugarcane, Ethephon, cDNA-AFLP, Differential gene expression,

摘 要 ：在甘蔗生长前期分别叶面喷施200 mg/L乙烯利和清水，利用cDNA-AFLP技术分析甘蔗在两种不同处理后体内基因表达差异情况，以期通过对差异表达基因进行序列分析和功能分析，探讨乙烯利调控甘蔗生长的分子机理。结果表明：经乙烯利处理后的甘蔗叶片，cDNA-AFLP产物多态性丰富，基因表达差异显著；部分差异片断序列与抗病、抗逆及促进光合作用等相关基因具有较高的同源性。

Abstract:A study was conducted to investigate differential gene expression in sugarcane (ROC16) leaves subjected to 200 mg/L ethephon. cDNA-AFLP amplification showed a high level of polymorphism，and gene expression was significantly different compared with control. Some sequences of different expression fragments were highly homologous to disease-resistant and photosynthesis-associated genes.

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