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作 者 ：魏冬霞1,2,3；高凯4；袁燕波1；汤正娇1；于晓南1,2,3*

期 刊 ：植物研究 2016年 36卷 2期 页码：190-194

关键词：芍药；胚培养；不定芽；

Keywords:Herbaceous peony, embryo-culture, root initiation,

摘 要 ：通过对芍药幼胚离体培养，可以有效缩短芍药的育种周期。本文以‘粉玉奴’、‘朱砂判’、‘团叶红’三个芍药品种不同采收期的种子为材料，研究不同胚发育时期、不同外殖体类型、消毒方式及启动培养基配方对芍药不定芽诱导的影响。结果表明，‘朱砂判’和‘团叶红’较适合胚培养成苗的发育时期分别为花后50和70 d；‘朱砂判’的合子胚相比于带胚乳胚，更适于启动和成苗培养；外殖体消毒的最佳方法为将心皮用70%酒精处理30~60 s，再用2%NaClO消毒20 min，之后取出胚珠用2%NaClO处理10 min；适宜的启动培养基配方为1/2MS+1.0 mg·L^-1 IAA+0.5 mg·L^-1 GA₃。

Abstract:Culture of zygotic embryos is an effective way to accelerate the breeding process of herbaceous peony. With the zygotic embryos of three herbaceous peony(Paeonia lactiflora Pall.) cultivars(‘Fen Yu Nu’, ‘Zhu Sha Pan’ and ‘Tuan Ye Hong’), we studied the affection of different embryo age, explant types, sterilization methods and starting medium formula on root initiation in vitro. The suitable development period were 50 and 70 d after flowering for embryo-culture of ‘Zhu Sha Pan’ and ‘Tuan Ye Hong’, respectively. We got better results when using excised zygotic embryos rather than keeping part of endosperm reserved. The optimal disinfection method for explant was that firstly treating carpel for 30-60 s with 70% alcohol, then for 20 min with 2% NaClO, and finally for 10 min with 2% NaClO. The optimal initial medium was 1/2MS+1.0 mg·L^-1 IAA+0.5 mg·L^-1 GA₃.

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