---

作 者 ：赵连飞;陈凤丽;孙曦晓;杜新琦;赵春建*;姚欢欢;杨磊;汪贵斌;祖元刚

期 刊 ：植物研究 2014年 34卷 4期 页码：561-566

关键词：牡丹种壳；芍药苷；丹皮酚；微波提取；

Keywords:peony seed coats, Paeoniflorin, Paeonol, microwave-assisted extraction,

摘 要 ：以牡丹种壳为原料，采用微波辅助提取方法，以芍药苷和丹皮酚得率为指标，利用单因素实验对于影响牡丹种壳中芍药苷和丹皮酚得率的因素进行优化。得到的最佳提取工艺参数为：乙醇提取分数70%，液料比10∶1、微波功率230 W、微波时间10 min，浸泡时间1 h,提取次数1次，牡丹种壳芍药苷和丹皮酚的得率分别为2.88和0.52 mg·g^-1。同时，对于选取的微波辅助提取方法的稳定性，回收率和重现性进行验证，结果显示微波辅助提取方法是一个稳定的、可靠的提取方法。

Abstract:In this study, single factor experiment was used to optimize the main factors of microwave-assisted extraction for paeoniflorin and paeonol from tree peony seed coats. The optimum extraction conditions were as follows: ethanol extract fraction was 70%, liquid-solid ratio was 10∶1, microwave power was 230 W, microwave time was 10 min, soaking time was 1 h and extraction times was one. Under these conditions, the yields of paeoniflorin and paeonol were 2.88 and 0.52 mg·g^-1, respectively. Meanwhile, the proposed method was validated in stability, repeatability and recovery experiments and the proposed method was a stable and efficiency method.

---