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作 者 ：张建瑛；祁永会*；吕跃东；刘建明；邢亚娟

期 刊 ：植物研究 2015年 35卷 1期 页码：22-26

关键词：核桃楸；组织培养；褐化；再生体系；

Keywords:Juglans mandshurica Maxim., tissue culture, browning, regeneration system,

摘 要 ：核桃楸在组织培养过程中褐化现象严重，影响着核桃楸培养物的正常生长与增殖。建立了核桃楸腋芽再生体系，并通过改变培养条件、培养方式以及在培养基中添加抑褐剂等方法，对影响核桃楸组培物褐化的因子进行了研究。结果表明：1~2年生核桃楸腋芽作为外植体诱导率显著高于10年和30年腋芽，达到75.8%；褐化率显著低于其他外植体为56.7%。DKW+BA 2.0 mg·L^-1+IBA 0.1 mg·L^-1的培养基可以降低褐化率并且促进植株生长，褐化率降低10%左右。Na₂S₂O₃和AC能够有效地缓解褐化；PVP和VC对组织培养物的褐化影响不显著。接种后进行5 d的低温暗处理可以推迟褐化产生的时间，褐化率降低10%左右。在培养基中加入6 g·L^-1的琼脂褐化率为56.7%。将诱导出的核桃楸苗在DKW+BA 2.0 mg·L^-1+IBA 0.01 mg·L^-1培养基中进行继代培养，增殖系数达到14.4，苗高为2.33 cm。继代周期21与28 d差异不显著，继代周期在21~28 d适合增殖培养。

Abstract:Browning takes place generally in tissue culture of Juglans mandshurica Maxim. and influences the growth and proliferation of cultures. We established the regeneration system for axillary bud of J.mandshurica Maxim. and studied the factors influencing the browning of cultures by changing culture environment and adding anti-browning agents into the culture medium. The inducing rate of 1-2 year axillary bud as explant was 75.8%, significantly higher than those of 10 and 30 year axillary buds. Browning rate was 56.7%, which was significantly lower than the others. DKW+BA 2.0 mg·L^-1+IBA 0.1 mg·L^-1 culture medium reduced browning and promoted plant growth, and the browning rate was lowered by about 10%. Na₂S₂O₃ and AC effectively alleviated the browning, while the alleviated effect of PVP and VC on browning was not significant. Fiveday dark treatment after inoculation delayed browning time, and the browning rate reduced by about 10%. Adding 6 g·L^-1 agar in culture medium resulted in browning rate of 56.7%. After subculture on DKW+BA 2.0 mg·L^-1+IBA 0.01 mg·L^-1 culture medium, the proliferation coefficient of seedlings was 14.4, and the seedling height was 2.33 cm. There was no significant difference between subculture cycles of 21 and 28 days, and a subculture cycle of 21-28 days was suitable for proliferation culture.

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