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作 者 ：孙晓莉;刘帅华;汪雷;李汶罡;赵春建*;祖元刚

期 刊 ：植物研究 2011年 31卷 (3)期 页码：377-380

关键词：绿茶；儿茶素；HPLC；

Keywords:green tea, catechins, high performance liquid chromatography(HPLC),

摘 要 ：建立了同时测定五指山绿茶中表儿茶素(EC)、儿茶素(C)、表没食子儿茶素没食子酸酯(EGCG)、表儿茶素没食子酸(ECG)和表没食子儿茶素(EGC)的高效液相色谱法。色谱条件为：HIQ SIL C18 V色谱柱(250 mm×4.6 mm，5 μm)；乙腈-1.5%冰乙酸体系为流动相进行梯度洗脱；紫外检测器，检测波长280 nm；流速为1.0 mL·min^-1；进样量10 μL；柱温为35℃。在优化条件下，5种儿茶素类成分的浓度分别在15~300、10~200、10~200、20~400和10~200 μg·mL^-1与色谱峰面积之间的线性关系良好；平均回收率在96.20%~99.64%之间；RSD在1.12%~2.24%之间。该方法简便、快速、准确，可用于绿茶中儿茶素的质量控制。

Abstract:A high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of epicatechin (EC), catechin (C), epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and epigallocatechin (EGC) in Wuzhishan green tea. The separation was performed on a HIQ SIL C18 V column (250 mm×4.6 mm, 5 μm) using gradient elution with acetonitrile-1.5% acetic acid as the mobile phase. The sample was detected by an UV detector at 280 nm, flow rate was 1.0 mL·min^-1, the sample volume was 10 μL and column temperature was 35℃. Under the optimized conditions, there was good linearity between the logarithm values and the peak areas of EC, C, EGCG, ECG and EGC in the ranges of 15-300, 10-200, 10-200, 20-400 and 10-200 μg·mL^-1, respectively. Recoveries of the five components were between 96.20% and 99.64%, the RSDs were between 1.12% and 2.24%. This method is simple, rapid, accurate and suitable for the quality control of green tea.

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