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作 者 ：陶能国;张继红

期 刊 ：植物研究 2008年 28卷 5期 页码：579-583

关键词：RT-PCR；柑橘；辣椒红／辣椒玉红素合成酶；基因；

Keywords:RT-PCR, citrus, Capsanthin/Capsorubin synthase, gene,

摘 要 ：利用RT-PCR技术从纽荷尔脐橙果实cDNA中分离出辣椒红／辣椒玉红素合成酶（Capsanthin/Capsorubin synthase，CCS）基因（Ccs）全长。该片断长为1619bp，编码503个氨基酸。BLAST比较其氨基酸同源性发现，该序列所推导的氨基酸与已知的甜橙CCS完全一致，与胡萝卜和辣椒的CCS，以及马铃薯新黄质合成酶（neoxanthin synthase，NSY）有70％的同源性。此外，与番茄和柑橘等的番茄红素环化酶也有50%~70%的同源性。半定量RT-PCR分析表明，Ccs在纽荷尔脐橙果实成熟过程中呈先上升后下降的表达趋势，期间9月份的表达量最高。

Abstract:The mRNA sequence for Capsanthin/Capsorubin synthase (CCS) has been cloned from fruits of Newhall navel orange by RT-PCR. Sequencing results revealed that it was 1 619 bp in length, encoding 503 amino acids. BLAST results showed that the deduced amino acids shared the same amino acid sequence as that of Citrus sinensis, 70% identities with CCS of Daucus carota and Capsicum annuum. In addition to 70% identities with neoxanthin synthase (NSY) of Solanum tuberosum, it showed 50%~70% identities with lycopene cyclase of Lycopersicon esculentum and citrus. Semi-quantitative RT-PCR results showed that expressions of Ccs were down-regulated during fruit ripening of Newhall navel orange, with the highest one in September.

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