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作 者 ：彭霄;段明慧;潘有智;张东阳;段喜华;*

期 刊 ：植物研究 2012年 32卷 6期 页码：756-759

关键词：木豆根；染料木素；微波辅助提取；Box-Behnken设计；

Keywords:pigeon pea roots, genistein, microwave-assisted extraction, Box-Behnken design,

摘 要 ：以木豆根为原料，利用微波辅助提取技术进行提取，在单因素实验的基础上对提取条件进行了考察，根据BBD(Box-Behnken design)实验设计原理，采用3因素3水平的响应面分析法，以木豆根中主要异黄酮染料木素(genistein)为指标，对提取过程进行优化，得到最佳工艺参数为：提取温度为68℃，固液比为32∶1 mL·g^-1，乙醇浓度为78%，提取功率700 W，提取时间15 min。在最佳提取条件下染料木素的提取率可达到0.465±0.032 mg·g^-1。本研究对于微波提取技术的应用及木豆根的开发和利用都具有显著的意义。

Abstract:Based on the preliminary experiments, a three-variable, three-level Box-Behnken design was performed to study microwave-assisted extraction of genistein from the roots of pigeon pea. The optimum parameters were as follows: extraction temperature 68 ℃, liquid/solid ratio 32∶1 mL·g^-1, ethanol concentration 78%, extraction power 700 W and extraction time 15 min. Under the optimal conditions, the average extraction yield of genistein was 0.465±0.032 mg·g^-1. This study is significant for the application of microwave extraction technology and utilization of the pigeon pea root.

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