Abstract:Banana bract mosaic virus (BBrMV) is a quarantine pests issued by Chinese government. To prevent the spread of the pests, a real-time fluorescent RT-PCR method was established based on specific primers and TaqMan probe which designed with the conservative sequences of the coat protein genes of different BBrMV isolates. Specificity study showed that typical amplification curves could be obtained for the two BBrMV strains, with Ct values of 26.65 and 27.52; and no typical amplification curve obtained for Potato Y virus, Plum pox virus, Prunus necrotic ringspot virus, Peach rosette mosaic virus and other viruses. The sensitivity of this method was 1000 times higher than of regular RT-PCR. Thus, the real-time fluorescent RT-PCR is a rapid, sensitive and highly specific method for the detection of BBrMV.