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期 刊 ：植物保护学报 2009年

关键词：李属坏死环斑病毒(PNRSV)；RT-PCR；外壳蛋白(CP)基因；系统发生树分析；

Keywords:Prunus necrotic ringspot virus (PNRSV), RT-PCR, coat protein (CP) gene, phylogenetic analysis,

摘 要 ：采用RT-PCR方法,从北京昌平、云南昆明、陕西西安和浙江海宁的樱桃和月季罹病叶片中检测到李属坏死环斑病毒,为分析我国李属坏死环斑病毒分子生态学特性,克隆了病毒分离物的CP基因并进行序列分析。结果显示,北京分离物的CP基因长675nt、编码224aa,而西安、昆明和海宁分离物的CP基因均为681nt、编码226aa。序列相似性分析表明,4个分离物的CP基因之间具 有很高的同源性, 各个分离物间的核苷酸同源性在94.8%~99.3%之间, 氨基酸同源性在94.2%~98.7%之间。序列比对与系统发生树的分析结果显示,北京分离物属于GroupⅡ组,昆明、西安和海宁分离物属于Group Ⅰ组。

Abstract:Four isolates of Prunus necrotic ringspot virus (PNRSV) coat protein (CP) gene were cloned from cherries in Changping (Beijing) and Xi’an (Shaanxi), roses in Kunming (Yunnan) and Haining (Zhejiang) by RT-PCR to analysis their molecular ecology characteristics. Sequence analysis results indicated that the CP gene of Beijing isolate consists of 675 nucleotides (nt) encoding of 224 amino acids residues (aa), and other three isolates from Xi’an, Kunming and Haining consist of 681nt encoding of 226 aa. High sequence identities were showed among four isolates of CP gene with the nucleotide sequence identities between 94.8% and 99.3% and the amino acid sequence identities between 94.2% and 98.7%. It is suggested that Beijing isolate belongs to GroupⅡ, Xi’an, Kunming and Haining isolates belong to Group I after aligned them with the sequences in GenBank and phylogenetic analysis.

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