Abstract:Rice variety Kongyu 131 was treated with pobenazole by root-irrigation, and the expressed proteins of rice leaves between the induced group and the concurrent control were analyzed using two-dimensional electrophoresis (2-DE). Through comparison of 2-DE maps between the two groups which was analyzed by PDQuest 8.0.1, at least ten up-regulated proteins (over two times than the concurrent control) were obtained, eight of them were identified with mass spectrometry. It was shown that they were well characterized including ubiquinol-cytochrome creductase, threonine endopeptidase, malate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, ribulose bisphosphate carboxylase, alternative oxidase, ascorbate peroxidase and plastid-specific 30S ribosomal proteins. Of which, the P1, P3 and P4 protein participate in respiration metabolism of plants; P2 protein might participate in degradation of protein; P5 protein could participate in synthesis of sugar; P6 and P7 protein participate in regulation balance of active oxygen metabolism. Analysis of differential proteins showed that probenazole induced rice resistance through increasing respiration of rice, degrading the proteins, promoting synthesis of resistance-associated sugar and scavenging of active oxygen.