全 文 :书!"#$%&
,2016,36(7):1302-1307
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
!"#$:10004025(2016)07130206 犱狅犻:10.7606/j.issn.10004025.2016.07.1302
%&(
:20160425;)*&%+(:20160525
,-./
:
()*+%,-
(30900971);./012%3456789+:;<=>(YJ201313)
0123
:
?@A
(1974-),B,CD,EFGHIJKLM$NO:P。Email:gaocanhong@163.com
QRST:UVW,CD,XYZ,EFGH[\#$]L;^5M$NO_]:P。Email:zhangshm893@sohu.com
456789:;<=>,?犅狅犆犢犘83犃1
@ABCDEFG
!"#
1,
$%%
1,
&(
1,
)*+
1,
,-.
2,
/01
2,
234
1
(1./012%,` a230036;2bcd2ef01ghijkl,bc350003)
H I:犆犢犘83犃1,mnopqr`sftuvwx,m,yz{|}~JK]CDBY10,RACE
RTPCR
,犅狅犆犢犘83犃1,mv。y,m1509bp,502,,vP450
。
Q4PCR ¡犅狅犆犢犘83犃1,m¢£¤K]、£¤¥¦|§£¤¨r©ª«v¬V®。
¯°7±² ¬W
,BoCYP83A15³´µ¶·w¯¸¹。犅狅犆犢犘83犃1,m¢£¤K]ºv¥¦»¼½£
¤
:
¢}~JK]CDBY10v¾、¿、À3¥¦º¬V®Á¼ÂÃ,¢K]CDBY12u¬e¿>À>¾,¢
CDBY14uĬe¾>¿>À。MeJA§SA©ªÅÆÇ6È犅狅犆犢犘83犃1,m¬vɱ:Ê MeJA©ª
Ë
,犅狅犆犢犘83犃1,m¬Ì?ÍÎÏv1.9Ð,ÑËiÒ«Ó;ÊSA©ªË,犅狅犆犢犘83犃1,m¬V®ÔÕ
ÓÖ
,
¢6hÓÖÍÎÏv0.1Ð,ÑË×ØÙÌÍÎÏv¬V®。:P¬W,犅狅犆犢犘83犃1,m¢£¤}~J
K]uv¬»½£¤
,
Ú¬ÆÇÛ MeJASAÜÝÞ。}~J犅狅犆犢犘83犃1vßà§á4â_?opq
rv}~JãK]ä4¡ªå,æ
。
>JK
:
}~J
;
opqr
;犅狅犆犢犘83犃1;çèéê;Vë
LMFN$
:Q785;Q786 !OPQR: !"#R:
犆犾狅狀犻狀犵犪狀犱犈狓狆狉犲狊狊犻狅狀犃狀犪犾狔狊犻狊狅犳犛狌犾犳狅狉犪狆犺犪狀犲犛狔狀狋犺犲狊犻狊狉犲犾犪狋犲犱
犌犲狀犲犅狅犆犢犘83犃1犻狀犅狉狅犮犮狅犾犻
GAOCanhong1,DONGLili1,GUANXiaowan1,ZHAOLiangxia1,LINJuncheng2,
XUFule2,ZHANGShuiming1
(1AnhuiAgriculturalUniversity,Hefei230036,China;2FuzhouChaodaModernAgricultureDevelopmentCo.Ltd.,Fuzhou
350003,China)
犃犫狊狋狉犪犮狋:犆犢犘83犃1isakeygeneinsulforaphanebiosynthesisandmetabolism.Thefullengthof犅狅犆
犢犘83犃1wasobtainedfrombroccoliCDBY10bythemethodsofRACEandRTPCR.犅狅犆犢犘83犃1was1
509bpinlength,encodedadeducedproteinof502aminoacids,andcontainedtheconservedP450domain.
Theexpressionlevelof犅狅犆犢犘83犃1indifferentcultivars,tissues,orseedlingstreatedwithdifferenthor
moneswasanalyzedbyrealtimefluorescencequantitativePCR.Phylogenetictreeanalysisshowedthat
BoCYP83A1hadtheclosestrelationshipwith犅狉犪狊狊犻犮犪狅犾犲狉犪犮犲犪var.狅犾犲狉犪犮犲犪BoCYP83A1.Tissuespecific
expressionof犅狅犆犢犘83犃1incultivarswasdifferent:expressiondifferenceinCDBY10wassmal.It
showedstem>leaf>rootinCDBY12,whileroot>stem >leafinCDBY14.ThetreatmentsofMeJA
andSAledtothechangesof犅狅犆犢犘83犃1expression:aftertheMeJAtreatment,theexpressionof犅狅犆
犢犘83犃1wasraisedto1.9timesofthecontrol,andthendecreasedalittle;AftertheSAtreatment,theex
pressionof犅狅犆犢犘83犃1reducedquickly,andupto0.1timeofthecontrolat6h,thenrestoredgradualy
totheexpressionlevelofcontrol.Theresultsindicatedthattheexpressioncharacteristicsof犅狅犆犢犘83犃1
inbroccolicultivarsweredifferent,and犅狅犆犢犘83犃1expressioncanberegulatedbyMeJAandSA.Clo
ningandcharacterizationof犅狅犆犢犘83犃1geneprovidethetheoreticalbasisforbreedingnewbroccoliculti
varswithhighsulforaphanecontent.
犓犲狔狑狅狉犱狊:broccoli;sulforaphane;犅狅犆犢犘83犃1;MeJA;SA
} ~ J (犅狉犪狊狊犻犮犪狅犾犲狉犪犮犲犪.var.犻狋犪犾犻犮犪
Planch)ìíîïðñiòóô,ng(7õ
¸2vIJö÷
,
Ûø
“
IJùú
”[1]。
}~J
¸ñ»ûvnïüÆs
———
opqr
(Sul
foraphane,SF),ysñiýþvÿ7!"$#
$
、
%&"(Mvüó
,
¢")*+
、
u+
、
,-
+ÅÆg.S
,
n/01¢IJugev2"
3½¸þv4*3½s
[23]。
567M$%v
gh
,
,m89:;}~J_]vã
=
。
opqr>1992?¢}~JuÛge[4],n@
ABCqD
(Aliphaticglucosinolates)4é,EF
GH,qD
(Glucoraphanin,RAA)Ó#Msv÷
]¼qIJ
。
:P¬W
,
opqrv`sftK
LÂMN
,
EF5O;Ps。QR`s*S
T$éq
,
5Ë|éqÈUV$
,
ÊW
XvYZ
、
qD[\v]sWXv^_`a
b3O;]sRAA。¸Ë,RAA¢cd7eS
«#sopqr
[57]。
fM$9g§犎犕犜、
犕犃犕、犆犢犘79/犆犢犘83、犃犗犘 bh`s,m(
C
[6]。
i犅狅犎犕犜 ©>opqr`sftKLv
¸*S
,
ÎéqvV®ÈwxÝÞS
[5];
犕犃犕1n犕犃犕 ,m(CuÞ&V$éqW
XYZ
,
]sjXqDkT$v,m
[5];犆犢犘79/
犆犢犘83,m(CnqD[\]svwx,m,
Úu犆犢犘79犉1l±`sXqD[6],犆犢犘83犃1Ä
ÝÞqD=ABmqD`s
;
qDWX^_`a
9uEFn 犃犗犘 ,m(CÝÞ,Úu 犃犗犘2
犃犗犘3ol±]spq,qDrs,qD[7]。
犆犢犘83犃1,mn(ûrt±eP450,m(C
ö÷
[8],
>*¢uvd
[911]、
wJ
[12]、
ÃxJ
[13]、
!
y~
[14]
b h ] # $ u Û á 4
。
z u v d u
犆犢犘83犃1,m¢xJu¬,xJuABC
d7wD{d7wDo|}4.52
Ð
[15]。
!y~犆犢犘83犃1,m¢uvdu¬
,
~,m#uABCd7wD 4MSOB
4MTBÅiWÌ,ðABCd7wD{Ì
?
[14]。
zJuv犅犼狌犆犢犘83犃11¢uvdu
¬6È¡ABCd7wDv
,
{À
d7w
D|}1.41~1.78Ð,~,m#uABCd7
wD|} 1.53~1.96 Ð[16]。`:P,
犆犢犘83犃1,mv¬ÆÇ6ÈABCd7w
D{d7wDv|}
,
Ñopqrv]s|
ABCd7wDv#*<
,
mfABCd7w
DvÆ6ÈopqrvÌ?
。
:P RACE
G}~Jußà¡
犅狅犆犢犘83犃1,mv, ¡ 犅狅犆
犢犘83犃1¢£¤K]、£¤¥¦º§ MeJA SA
¨r©ª«v¬»½
。
y:P£7÷
:P犅狅犆犢犘83犃1v7M$%üÆä4,æ,
?opqrvãK]â_<¡,m
。
1 5
1.1 STUV
3opqr£¤v}~JK]CD
BY10、CDBY12CDBY14z{,h}
~J£¤K]
、
£¤¥¦º,m¬Á¼v:P
。
z3K]>128u,K]10
,3_M。 _¡¢ªÍ3À1\£¤。
oz¤÷Mv10¾、¿、À3]¥¦£¤,
ÔÕ§¨Õ©Ë~ª-80℃«¬®¯。
|CDBY12z{,h°^¨rÝÞ«
,mv¬Á¼:P
。
±²âzCDBY12
]>g³´
(10cm×10cm)u,5mLµ¶Vz
·²¸¹
,
*Ëi]100º|»¼½÷!,
©ª]3M。¾g³5d,o
1mmol/LMeJA3mmol/LSA¿À©ª,|¿
Àµ¶Vv©ªÎÏ
,
¿ÀÞ&÷!
。
¿À©
ªË3、69hÁ£¤1_,ÔÕª§¨Õ©,Ë
~ª-80℃¯。
1.2 W X
1.2.1 ,?YZ[\@AB ±TrizolÃ<
30317+ ?@A,b:}~Jopqr`sÄw,m犅狅犆犢犘83犃1vßà5¬
}~JÀ
v{ RNA,ÅSMARTTM RACE
cDNAAmplicationKit(Clontech)7¼Æ~Ç
cDNA。¾ÈNCBIÉÊÇvuvd、wJ、op
b$]v犆犢犘83犃1ËÌ6$83PF83
PR,7¼,||uº
;。¾ÈvË
Ì3’=6$F3′1、F3′2Æ=6$R5′1、R5′2,
5Ë5Í46$ UPM、NUP7¼5′3′ÎSv,
|
。
zÏÐ7¼ÑÒ,mv
。
ËÌ
6$83HSF83HSR,?ÓePrim
erSTARHS7¼PCR,|ÆÔ,|ÎÑv
7¼Õ
。
1.2.2 ,?]^_`aFGbcdefg@hi
DNAMAN2.67¼£¤$]ºÖxv
×Î
;
±Ø¢ MEGAuÙ¯°g_²,Ë
ÚBootstrapM 500_。SignalP7¼
RÛÜÝÞ
,
PDB7¼Pfamdomain Motif
ÝÞ
。
1.2.3 ,?DEFG ±TRzol(Invitrogen)
Ã<¤Kv{RNA,ÅDNaseⅠ(TaKaRa)
zßàvDNAᱩª,5Ë£2μgRNAÅ
PrimeScript RTreagentkit(Takara)7¼Æ~
Ç
。
¾ÈÉv犅狅犆犢犘83犃1,mËÌ6$
83RTF 83RTR,| 18S â ã , m,ä Ï
SYBRPremixExTaqTMⅡ (Takara)zå´v
ñæçSè¢ABI75004é7¼,|,
ÄάvÌêÅ2-ΔΔCT
。¥¦»¼½¬
D1 jST=>@k^[Table1 Relatedprimersequencesof犅狅犆犢犘83犃1
intheexperiment
6$ëì
Primername
6$
Primersequence(5′→3′)
83PF ATGGAAGATATCATCATCGG
83PR GAACGGTATAAACTCGTAGT
UPM
CTAATACGACTCACTATAGGGCAAG
CAGTGGTATCAACGCAGAGT
CTAATACGACTCACTATAGGGC
NUP AAGCAGTGGTATCAACGCAGAGT
F3′1 ACAGGTCTCACGGCTTATAT
F3′2 TAGAGAATGTCAAAGGCGTG
R5′1 CATTCCCATCTTCCTCATCT
R5′2 CCACCATTGTTTGACTTCCT
83HSF ATGGAAGATATCATCATCGG
83HFR TTATTTGCTCACTTTCTCCG
83RTF CTTCAAAGGCACGGACTA
83RTR AACGGAACCTCAAGCATCG
|À
uv¬ÎÏ
,
£¤¨r©ªu
|CKv¬SÎÏ。
íȱExcel2003、DPS7.05、Agilent1100
offlinestudioî7¼°Ì 。
2 ô5
2.1 456犅狅犆犢犘83犃1,?犮犇犖犃YZ@AB
|83PF/83PR6$7¼PCR,|,
犅狅犆犢犘83犃1vuº
; (ï1,A)。Å F3′1/
UPM、R5′1/UPM6$7¼ð÷ñvPCR,|Æ
Ô
,
|ÆÔT$Sòó
,
oF3′2/NUP、R5′2/
NUP6$7¼ð^ñvPCR,|ÆÔ,750
bpôõv3′
; (ï1,C)260bpôõv5′
;
(
ï
,1,B)。zÏÐ7¼ÑÒ1630bp
。
5Ë|83HSF/83HSR6$,Å?
Óe7¼,|
,
|ÎÑv7¼Õ
,
|f
ö4}~Ju犆犢犘83犃1vcDNA(ï1,
D)。 NCBIv ORFFinder7¼ ,}~J
犆犢犘83犃1,m1509bp,502,
(
ï2),÷ë犅狅犆犢犘83犃1。
2.2 犅狅犆犢犘83犃1,?@[\befgFG
z犅狅犆犢犘83犃1,mv,5Úø
$]v,7¼×Î
,
geBoCYP83A15
wJBnCYP83A1vÄù½¸?,Ò97.01%。
5uvdAtCYP83A1、opRsCYP83A1Äù½
o87.67%96.02%。
ESyPred3D¢úÝÞBoCYP83A1Öx
LÏû
,
Ú?üñi H x306,ýþí
20,~ÿí44,ÖxL757.52kD,b
M.DL2000;A.uº
;T$;B.5’RACET$;
C.3’RACET$;D.cDNA
ï1 犅狅犆犢犘83犃1,mv,|ô
M.DL2000;A.Internalfragment;B.5’RACEfragment;
C.3’RACEfragment,D.ThefullengthcDNA
Fig.1 PCRamplificationresultsof犅狅犆犢犘83犃1
4031 ! " # $ % & 36!
"#7.96。QSignalPNNÝÞyÖx¢ð25~
26,v$Úñi1RÛÜ。Q
PfamíÈ%×Î ,BoCYP83A1Öxö
P450(C,m,&ð31~494,,
ðð4~21,1&(Äw(ï2)。
¡ö4BoCYP83A15Úø$]CYP83A1
v¶·w¯
,
MEGA5.05 î,z BoC
YP83A15Éßàvuvd、wJ、op、´ µ、dJ
E)b6$]vCYP83A1s7¼h
×ί°7±
。
ô*
,BoCYP83A1
´µBoCYP83A1(2)v¶·w¯¸¹,+÷,,
Ñ5uvdAtCYP83A1E)CsCYP83A1¶
·w¯Â-
(
ï3)。
2.3 犅狅犆犢犘83犃1,?lmnopmnqrs@D
Etu
}~J犅狅犆犢犘83犃1,m¢£¤K]v
¾
、
¿
、
À¥¦uv¬V®
,
ôiï4Ü*。Ú
u
,犅狅犆犢犘83犃1,m¢CDBY10v3¥¦º¬
V®Á¼ÂÃ
,
¢CDBY12u¬e¿>À>
¾
,
Ñ¢CDBY14uĬe¾>¿>À。¢¾¯
u
,CDBY14犅狅犆犢犘83犃1,mv¬.?>
Ú/K]
。
¢¿u
,CDBY14犅狅犆犢犘83犃1,mv
¬V®ÄÎÚøK]Â?
,
ÄÁÂÃ
;
Ѣu
,CDBY10犅狅犆犢犘83犃1,mv¬?>
CDBY14CDBY12。
2.4 v:wxy犅狅犆犢犘83犃1,?@DEtu
Σ¤¨r£¤º©ªË
,犅狅犆犢犘83犃1,
m¬7¼0Þge
:
Ê MeJA©ª3h,犅狅
犆犢犘83犃1,m¬Ì?ÍÎÏv1.9Ð;56
ºv12
,犅狅犆犢犘83犃1,m¬V®¢69h
iÒ«Ó
,
3?>ÎÏ
。
ÊSA ©ª3h,
犅狅犆犢犘83犃1,m¬V®ÓÖ,ð¢6hÒ¸
Ö
,
ÎÏv0.1Ð,Ñ9hiÜÙÌ,ð4?>
CKv¬V®(ï5)。
AtCYP83A1.uvd (NP_193113);BnCYP83A1.wJ (NP_001303017);RsCYP83A1.op(AHB11194.1);
ïucû
、
Aû§µûof¬,P
、
ЧÄù
。
ï2 犅狅犆犢犘83犃1,m14v,5Úø$]CYP83A1sv×Î
AtCYP83A1.犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪(NP_193113);BnCYP83A1.犅狉犪狊狊犻犮犪狀犪狆狌狊(NP_001303017);RsCYP83A1.犚犪狆犺犪狀狌狊狊犪狋犻狏狌狊
(AHB11194.1);Aminoacidshighlightedinblack,redandblue,respectivelyrepresentresiduescompletelyconsewed,partialyconservedand
similartoconsensus.
Fig.2 Alignmentofthepredictedaminoacidsequencesof犅狅犆犢犘83犃1withotherCYP83A1s
50317+ ?@A,b:}~Jopqr`sÄw,m犅狅犆犢犘83犃1vßà5¬
£¤$]vCYP83A1ÖxÊÇÛi«:BoCYP83A1(2).´µ
(XP_013636864);BnCYP83A1.wJ (NP_001303017);RsCYP83A1.
op
(AHB11194.1);BjCYP83A1.J (AGO37757);AtCYP83A1.
uvd
(NP_193113)CsCYP83A1.E) (XP_010495830)
ï3 £¤$]CYP83A1sv¯°7±
CYP83A1proteinaccessionnumbersfordifferentspeciesareas
folows:BoCYP83A1(2).犅狉犪狊狊犻犮犪狅犾犲狉犪犮犲犪var.狅犾犲狉犪犮犲犪
(XP_013636864);BnCYP83A1.犅狉犪狊狊犻犮犪狀犪狆狌狊(NP_001303017);
RsCYP83A1.犚犪狆犺犪狀狌狊狊犪狋犻狏狌狊(AHB11194.1);BjCYP83A1.
犅狉犪狊狊犻犮犪犼狌狀犮犲犪(AGO37757);AtCYP83A1.犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪
(NP_193113)CsCYP83A1.犆犪犿犲犾犻狀犪狊犪狋犻狏犪(XP_010495830)
Fig.3 PhylogeneticanalysisamongdifferentCYP83A1s
£¤Ã567¬*£¤K]}~J8¡¥¦º,m
¬¢0.05V®Á¼.½
ï4 £¤K]}~J8¡¥¦º犅狅犆犢犘83犃1
,m¬Á¼
Normallettersindicatesignificantdifferenceof犅狅犆犢犘83犃1
expressionamongdifferentcultivarsortissuesofbroccoli
at0.05level
Fig.4 Expressiondifferencesof犅狅犆犢犘83犃1gene
amongdifferenttissuesorcultivarsofbroccoli
3 9 å
:PG}~Jußà¡犆犢犘83犃1v¤^,
m
,
uvP450üÆ,mf÷ë
犅狅犆犢犘83犃1(ÊÇÛKU573063)。¯°7±²
*
:BoCYP83A15Úø$]vCYP83A1È
^Ĥ
,
önCYP83A1(Cv¤^,m,¢Ë
:7±v£¤+
,
5Úø$];:
。BoC
YP83A15³´µBoCYP83A1(2)v¶·w¯¸
£¤Ã567¬*£¤¨r£¤©ªº
¢0.05V®vÁ¼.½
ï5 £¤¨r£¤º©ª«犅狅犆犢犘83犃1
,m¬Á¼
Normallettersindicatesignificantdifferenceanomgtreatments
withdifferenthormonesandtimesat0.05level
Fig.5 Expressiondifferencesof犅狅犆犢犘83犃1geneof
broccolitreatedwithdifferenthormonesandtimes
¹
,
+÷,
,
Ñ5uvd AtCYP83A1E)
CsCYP83A1¶·w¯Â-。
£3}~JK] CDBY10、CDBY12
CDBY14,ÎÚ£¤¥¦uv犅狅犆犢犘83犃1¬
7¼0Þ
。CDBY14u犅狅犆犢犘83犃1v¬V®
W?>CDBY10CDBY12。£¤K]u犅狅
犆犢犘83犃1v ¥ ¦ » ¼ ½ ¬ £ Ä ¤:犅狅犆
犢犘83犃1¢CDBY103¥¦º¬V®Á¼Â
Ã
,
¢CDBY12u¬e¿>À>¾,Ñ¢CDBY
14uĬe¾>¿>À。
。
:Pge
,
J
犅犼狌犆犢犘83犃11、犅犼狌犆犢犘83犃12、犅犼狌犆犢犘83犃13
犅犼狌犆犢犘83犃14b4,mv¬Å¬e>¿
>À>¾v?@[16];ABu犆犢犘83犃1v¬»½
À>¾>ÀC[13]。D5犅狅犆犢犘83犃1¢}~J
CDBY12v¬Äù。
犆犢犘83犃1,mv¬Æn°^¨rÜÝÞ,
iJ
,
¢ MeJAEF«,G犅犼狌犆犢犘83犃11¬
ɱ£.
,犅犼狌犆犢犘83犃12、犅犼狌犆犢犘83犃13
犅犼狌犆犢犘83犃14¬ÅWÝ;¢SA EF«,
犅犼狌犆犢犘83犃11、犅犼狌犆犢犘83犃12、犅犼狌犆犢犘83犃13
犅犼狌犆犢犘83犃14b4,mv¬ÅW«
Ý
[16]。
:Pôge
,MeJA¿À3hË,}~J
犅狅犆犢犘83犃1¬V®.,6~9h¬
iÜ«Ó
,
3H?>ÎÏ
。SA¿À3hË,犅狅犆
犢犘83犃1¬V®.ÓÖ,¢6hÓÖÎÏ
6031 ! " # $ % & 36!
v0.1Ð,9hÄÙÌÍÎÏV®。@f1Þ MeJA
SAÆÇÝÞ}~J犅狅犆犢犘83犃1v¬。
Ü
,
}~J犅狅犆犢犘83犃1,mv¬5
ÝÞñi÷4v½
。
ËI:PzÎ 犅狅犆
犢犘83犃1,mv7M$%üÆ7¼J# ,G
Ñâ_?opqrv}~JãK]<ªå
KÈ,m;^
。
z{!O
:
[1] UL.}~JML?TNâNO[M]."O:-P>QR,
2007:17.
[2] STU,VWX,?@A,b.}~Jq、YftZ[§ÚÎ
òüÆv\]:P7h
[J].u(M$%%&,2011,33
(4):422432.
LINJC,WUQY,GAOCH,犲狋犪犾.Thecompetitionofsul
furandseliniumandtheireffectiononhealthcarefunctionin
broccoli:areview[J].犆犺犻狀犲狊犲犑狅狌狉狀犪犾狅犳 犆犲犾犾犅犻狅犾狅犵狔,
2011,33(4):422432.
[3] MOHAMEDAF,AMIRAA M.Sulforaphanecomposition,
cytotoxicandantioxidantactivityofcrucifervegetables[J].
犑狅狌狉狀犪犾狅犳犃犱狏犪狀犮犲犱犚犲狊犲犪狉犮犺,2010,1:6570.
[4] ZHANGYS,CHOCG,GARYHP,犲狋犪犾.Spectroscopic
quantitationoforganicisothiocyanatesbycyclocondensation
withvicinaldithiols[J].犃狀犪犾.犅犻狅犮犺犲犿.,1992,205(1):
100107.
[5] GAOC,LINJ,ZHAOL,犲狋犪犾.HomocysteineSmethyl
transferaseregulatessulforaphaneconcentrationin犅狉犪狊狊犻犮犪狅犾
犲狉犪犮犲犪var.犻狋犪犾犻犮犪[J].犐狀犱犻犪狀犑狅狌狉狀犪犾狅犳犌犲狀犲狋犻犮狊犪狀犱犘犾犪狀狋
犅狉犲犲犱犻狀犵,2015,75(3):357362.
[6] SONDERBYIE,GEUFLORESF,HALKIERBA.Biosyn
thesisofglucosinolatesgenediscoveryandbeyond[J].犜狉犲狀犱狊
犻狀犘犾犪狀狋犛犮犻犲狀犮犲,2010,15(5):283290.
[7] WANGH,WUJ,SUNS,犲狋犪犾.Glucosinolatebiosynthetic
genesin犅狉犪狊狊犻犮犪狉犪狆犪[J].犌犲狀犲,2011,487(2):135142.
[8] ZANGYX,KIMHU,KIMJA,犲狋犪犾.Genomewideidenti
ficationofglucosinolatesynthesisgenesin犅狉犪狊狊犻犮犪狉犪狆犪[J].
犉犈犅犛犑狅狌狉狀犪犾,2009,276(13):35593574.
[9] NAURP,PETERSEN BL,MIKKELSEN M D,犲狋犪犾.
CYP83A1andCYP83B1,twononredundantcytochromeP450
enzymesmetabolizingoximesinthebiosynthesisofglucosino
latesin犃狉犪犫犻犱狅狆狊犻狊[J].犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔,2003,133(1):63
72.
[10] HEMM MR,RUEGGERMO,CHAPPLEC.The犃狉犪犫犻
犱狅狆狊犻狊狉犲犳2 mutantis defectivein the gene encoding
CYP83A1andshowsbothphenylpropanoidandglucosinolate
phenotypes[J].犘犾犪狀狋犆犲犾犾,2003,15(1):179194.
[11] BAKS,FEYEREISENR.TheinvolvementoftwoP450en
zymes,CYP83B1andCYP83A1,inauxinhomeostasisand
glucosinolatebiosynthesis[J].犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔,2001,127
(1):108118.
[12] U[[.wJuvdu^qf_`aD`s§ÝÞ,m
vüÆ
[D].bc:du012%,2015.
[13] ZHUB,WANGZ,YANGJ,犲狋犪犾.Isolationandexpression
ofglucosinolatesynthesisgenesCYP83A1andCYP83B1in
pakchoi(犅狉犪狊狊犻犮犪狉犪狆犪 L.ssp.犮犺犻狀犲狀狊犻狊var.犮狅犿犿狌狀犻狊
(N.Tsen&SHLee)Hanelt)[J].犐狀狋犲狉狀犪狋犻狅狀犪犾犑狅狌狉狀犪犾狅犳
犕狅犾犲犮狌犾犪狉犛犮犻犲狀犮犲狊,2012,13(5):58325843.
[14] efªg.!y~CYP83A1,mvßà§üÆ{»[D].h
ij
:
k"012%
,2015.
[15] ZANGYX,KIMJH,PARKYD,犲狋犪犾.Metabolicengi
neeringofaliphaticglucosinolatesinChinesecabbageplants
expressing犃狉犪犫犻犱狅狆狊犻狊 MAM1,CYP79F1,andCYP83A1
[J].犅犕犅犚犲狆狅狉狋狊,2008,41(6):472478.
[16] AUGUSTINER,MAJEEM,PRADHANAK,犲狋犪犾.Ge
nomicorigin,expressiondifferentiationandregulationof
multiplegenesencodingCYP83A1,akeyenzymeforcore
glucosinolatebiosynthesis,fromthealotetraploid犅狉犪狊狊犻犮犪
犼狌狀犮犲犪[J].犘犾犪狀狋犪,2015,241(3):651665.
(
!"
:
#$%
)
70317+ ?@A,b:}~Jopqr`sÄw,m犅狅犆犢犘83犃1vßà5¬