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作 者 ：杨 宁 1,李翠霞 1,2,李志忠 2,张 继1*

期 刊 ：西北植物学报 2012年 2期 页码：330~335

关键词：百里香；苯丙氨酸解氨酶；诱导子；酶活性；

Keywords:Thymus, L-phenylalanine transaminase, elicitor, enzyme activity,

摘 要 ：以1/2MS为基本培养基，分别用蔗糖、NO₃^-/NH₄⁺、苯丙氨酸、谷氨酸和酪氨酸诱导培养百里香组培苗30 d，以及茉莉酸甲酯（MeJA）、水杨酸（SA）和壳聚糖诱导培养百里香20、30和40 d组培苗植株0～120 h，在不同的时间点测定其苯丙氨酸解氨酶（PAL）活性，以探讨诱导子对增殖百里香中PAL活性的影响。结果显示：以1/2 MS为基本培养基，碳氮源调控30 d后，百里香组培株的PAL活性以蔗糖浓度为2%、NO₃^-/NH₄⁺比例为2∶1时最高；前体调控添加0.6 mmol/L苯丙氨酸、0.8 mmol/L谷氨酸和0.4 mmol/L酪氨酸处理的百里香组培苗的PAL活性最高，分别为相应对照的126.3%、150.6%和153.9%。培养20 d和30 d植株的PAL活性均以1.0 mmol/L MeJA、100 mg/L SA和200 mg/L壳聚糖诱导处理的最高，分别为相应对照的610.3％、788.1％、592.5％以及402.4％、541.2％、400.1％；而40 d植株经0.5 mmol/L MeJA、100 mg/L SA和200 mg/L壳聚糖诱导的PAL活性最高，分别为对照的390.2％、377.1％和413.4％。可见，在不同时期添加不同的诱导子对百里香再生植株中PAL活性有显著影响，且不同材料和诱导子存在各自适宜的诱导浓度和最佳诱导时间。

Abstract:The plantlets of thyme were cultured on the 1/2 MS containing different sucrose,the NO₃^-/NH₄⁺,phenylalanine,glutamic acid and tyrosine,and were tested for the PAL activity.The PAL activities were also tested after the plantlets were induced for 0～120 h by MeJA,SA and chitosan at different culture times (20 d,30 d,40 d).The results showed that the highest PAL activity was obtained when the plantlets of thyme were cultured on the 1/2 MS medium with 2% sugar concentration and 2∶1 NO₃^-/NH₄⁺ ratio for 30 d.The PAL activities were increased to 126.3%,150.6% and 153.9% compared with CK by adding 0.6 mmol/L phenylalanine,0.8 mmol/L glutamic acid,and 0.4 mmol/L of tyrosine as precursor regulation,respectively.The PAL activities of plantlets cultured 20 d were increased 610.3％,788.1％ and 592.5％ than that of CK by adding 1.0 mmol/L MeJA,100 mg/L SA,and 200 mg/L chitosan as elicitors.The PAL activities of plantlets cultured 30 d were increased to 402.4％,541.2％ and 400.1％ compared with CK.And the highest PAL activities of plantlets cultured 40 d were increased to 390.2％,377.1％ and 413.4％ than that of CK.Therefore,the PAL activity was affected by adding different inducer at different culture time.

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