Abstract:In order to control the browning of tissue culture on Phalaenopsis, the contrast test of adsorbent: activated charcoal, PVP, and five antioxidants: citric acid, cysteine, glutathione, vitamin C, sodium thiosulfate in tissue culture of Phalaenopsis were studied. The results showed that activated charcoal can control browning and promote growth. But at the same time, it was adverse to the differentiation. Although the glutathione, which the effect of antibrowning was not as good as activated charcoal‘s, its resultant effect was the best one.