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Effects of Manganese and SA on Germination of Achnatherum inebrians Seed Containing or Free of Endophyte

锰、水杨酸与内生真菌互作对醉马草种子萌发的影响



全 文 :!24" !1#
 Vol.24  No.1
! " # $
ACTA AGRESTIA SINICA
   2016$  1%
  Jan.  2016
犱狅犻:10.11733/j.issn.10070435.2016.01.015
¾

ó¿qWÞcbR5$ÀÁŒe‘’2|u
w4q

, x,0yz,){v

-_()9\]^¬•­^CË ÈYY+-_()*¾+,

67 ÈY730020)
78

»/0<=íS,°BC

h"6@×AT MnÓùDÜ(E+)6Ü29—Þ(E-)É-(犃犮犺
狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊)Gmh°T)cdj|e”QƒW(salicylicacid,SA)D Mnú5TŒ”cd。efgh,
¼î MnÓùˆ¸,E+É-Gm°Ý6°»ë©…†(犘<0.05)séE-É-Gm,Àì MnÓù×A
TíY
,E+É-GmTh°Ý©…†€(犘<0.05),nE-É-Gm°Ý6°»ëغ;Àì
MnÓù×ATíî,E+6E-É-}Ÿ©„h…TœóI,nŸï…†Øº;Le”SAÜÓù×Aœ,
E+É-}Ÿ©…†(犘<0.05)séE-É-}Ÿ,L10mg·L-1MnÓùá&=¾¼î6@×ATe”SA,
ef…˜e”SAD MnÓùÞJŒ”cd。ÓS1ð,29—Þ»BUVÉ-GmTh°,•¸¬ú5DÉ
-Gmh°ÞJß©T)cd

ne”SALjK-_Œ”.ßú5。
9:;
:Mn;SA;É-;29—Þ;Gmh°
<=>?@
:S452    ABCDE:A     AFG@:10070435(2016)01010707
犈犳犳犲犮狋狊狅犳犕犪狀犵犪狀犲狊犲犪狀犱犛犃狅狀犌犲狉犿犻狀犪狋犻狅狀狅犳犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊
犛犲犲犱犆狅狀狋犪犻狀犻狀犵狅狉犉狉犲犲狅犳犈狀犱狅狆犺狔狋犲
WANZhiwen,WANGPing,ZHANGXingxu,LIChunjie
(KeyLaboratoryofGrasslandFarmingSystems,ColegeofPastoralAgricultureScienceandTechnology,
LanzhouUniversity,Lanzhou,GansuProvince730020,China)
犃犫狊狋狉犪犮狋:Variousconcentrationsofmanganese(Mn)andsalicylicacid (SA)affectedgerminationof
drunkenhorsegrass(犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊)seedsthatwereinfectedwith(E+)orfree(E-)ofa犈狆犻
犮犺犾狅犲/犖犲狅狋狔狆犺狅犱犻狌犿endophyte.TheresultsshowedthatE+specimensshowedasignificantly(犘<0.05)
highergerminationrateandindexthanthatofunderMnfreetreatment.ThegerminationrateofE+speci
mensshowedattendofdecreasingongerminationratewiththeincreasingofMnconcentration.Therewere
significantdifferencesexistedbetweentreatments(犘<0.05);butthegerminationrateandindexofE-
specimenshadnosignificantdifferences.E+specimensshowedasignificantly(犘<0.05)higherroot
lengthwhenvariousconcentrationsofexogenousSAwereadded.TheresultsshowedthatexogenousSA
couldrelieftheeffectsofMnonseedgermination.
犓犲狔狑狅狉犱狊:Mn;SA;犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊;Endophyte;Germination
HIJK
:20141224;LMJK:20151013
NOPQ

¬
”973”´ µ(2014CB138702);¬¶·*+®¸´µ(31372366);öJúÁÂSÊ°±²³´µ(IRT13019)“Å
5RST

u¹Š
(1992),È,67áQÉ,šË/09,/0Òcnq-29—Þt9N,Email:wanzhw14@lzu.edu.cn;<Ôc™
Authorforcorrespondence,Email:chunjie@lzu.edu.cn
  q-29—Þ(Fungalendophyte)Úßà§B
Lq-&A=àú$Yú=9ý¹#

I6ËÌ
JK…˜Geú;‰T—Þ
[1]。
µvþÀLû
&%Fq-&°P¡犖犲狅狋狔狆犺狅犱犻狌犿 ¬|‘J1
,犈狆犻犮犺犾狅ё¬T29—Þ[2],.à29—ÞT+L
kJKThi§¥J1í–

Ì«5n‘›s¡
ÌÌJKDÜG9K
[3]
6È9Kú5
[4]
TŽc

~øö
[56]、
ø‰
[78]、
ø
[910]、
Ž)
[1112]
6D•¸¬ú5
[13]
TŽc

É-
(犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊)Úq»*ÍÍ

(犃犮犺狀犪狋犺犲狉狌犿)$9-»JK,ÚÕ:Ò
á·-.Ì«T]Ú-ãß

LÂÜ

234


ª

‹Œ

67?ž

Ž

©J=>
[14]。1994$V
+™Bruehl?[15]Œ“LÔ¶ÕÂÜTÉ-G
m&°P¡29—Þ
。2000$Z¹F6eö|[16]
L67žÉ-2°P¡29—Þ 犖犲狅狋狔狆犺狅犱犻
! " # $ !24"
um,2004$eö|?[17]L67žÉ-2°P¡
29—ÞÂG犖.犵犪狀狊狌犲狀狊犲,}ÏÝÂw—Þý
ïâ£

P•Âýïn犈狆犻犮犺犾狅犲犵犪狀狊狌犲狀狊犻狊[18]。2
9—Þ›s¡É-Thi§¥
[19],
Ls×AT•
¸¬ú5œÉ-TŽcJß©T›s
[20]。
ÀìÃ)T°±

EŠTi>
[21]、

[22]

”
[23]
TY`d

•¸¬´µ
[24]
ò˜ô•

’n-
·Ó"TßYHl„þ
[25]。
jÜGïÂiOT•
¸¬ô•´µ¡ÙC6QF

LJK&Y`Ö¹T
•¸¬67ô•UV¡JK‡PT9Ÿ°J69ù
9ðoØ

nò]Lj<=&KàD!K6Éà²
³+’ß©ÄAT°±
[26]。
•¸¬ø
(Mn)ÚJK
9Ÿ1ò3T?`üu

žJKGÂtoØ=
Ä

Ì«cn‘šcd&øÁð½T™5m
[27]。
K
`T MnL[\Jr‡P9ŸÆíî¤`,I-×
AQ=ß©<=

ÿDJK¤9Ú±cd
[2829]。
Ï


QƒW
(salicylicacid,SA)LJKøHlú
5
[3031](
~&

p

)

š‰

•¸¬?

6±
[32]
Ò
ÓÞJh…T:f

LjŒ”•¸¬TÚ±
[33]
6)
ú5DGmh°|ÈÉT)cd
[34],
íîJrT
¼•69K`
[35]。
Gmh°2#T9Ÿ‰ö"#UVJKT9Ÿ
°J6¤`
[36],
5.

»BC•­/0L•¸¬ Mn
ÓùÍ֜܁6ÜÞÉ-GmTh°xö

W
XSAD•¸¬ú5TŒ”cd,n\ß/0É
-D•¸¬ú5Tø

9ù9ð|=mÁù0
©¡ß©®¯

1 UVWXY
1.1 jkUV
É-Gmé2011$Ô¶67á¯0Ït(E
102°52',N37°12)ÆbGéÈYY+Ð&˜Ž,
2012$10%_—TGm5℃Ð+é()ú?-
-„-GmF`|Ñ×C&!

ÈY

GmhŒË

2013$3%­ÜÞ(E+)66ÜÞ(E-)É-

,2014$7%Ð_—TÉ-Gmdé»B
C

1dBÜ MnCl2·4H2O、QƒW (salicylic
acid,SA)©j=•Úñâ¼î;QnA5mQ。
1.2 jkXY
1.2.1 Mnp[ESA”E+pE-|e>34}
~E_ý Mn2+Óùá×A=¾n0,50,100,200
mg·L-1,SA Ó ù á × A = ¾ n 0,5,10,15
mg·L-1;j¼î?`A5mQnDÒ。fgÓ
²³

h]W1ò’kAt`TGm

d5%T“X
WâÛẻ10min,jd¶çQ ¼ë“,Ud
°ôQ ¼3“,dìíÐQ^š。f"Ân9
cmT“D¹,¹2jY’ìín°,,Ж`T
•¸¬Ûáîƒ7mLÂé“D¹&。¿¹©Q>
ƒ30]É-Gm,¿MÓù3M•*,MNGm
Th°ë

æ}Gm?Ÿ

æŸAWdGmß
ˆ

34nGmþÀ°
)。GZ025Fà¶!‘Ò
“Dà&ÛAn
(20±1)℃,‘Ò2%n16D/8N。
°#%¿r24hMNߓ,!10d^²°Ý,
Ʋ߰»ë

°Ý
(%)=œBGmT°ë/œBGmë
×100。
°»ë
(犌犐)= ∑犌狋/犇狋,â&犌狋nL狋ò
T°ë
,犇狋n°áë。
1.2.2 [ESA” MnWXdE+pE|e>3
4€p‚E_ý GmÀ°ôQºG24h
_

bGé¼J2’ìíT“D¹,î10mg·L-1
Mn2+Óùá,LÓùá&¼î6@×A@ATSA,
SA×A=¾n0,5,10,15mg·L-1,>ÂéÎÛà
228±1℃Íh°,h°7d_^²æ}Ÿ6æ
Ÿ

1.2.3 Lƒ„ꌍ  1JëÏ©d Microsoft
Excel2003Nƒ,Æcë。ÔdSPSS16.0forwin
dows^²=•ÕÖ\‘غ…†=•,dDun
canâ\‘•¸t。
2 pqW>r
2.1 Mnno$E+wE-ÀÁŒe‘’2|u
èg1Lã,Àì MnÓù×ATíî,E+É
-Gm°Ý6°»ë©„h…TœóI

nE-É-Gm°Ý6°»ëغ。
-MnÓù×An200mg·L-12,E+É-Gm
°Ý…†
(犘<0.05)€é MnDÒ,€¡×
12%;50,100,200mg·L-1MnÓùœE+É-
Gm°»ë©…†
(犘<0.05)€é MnDÒ,=
¾€¡×8%,9%615%;L MnDҜ,E+É
-Gm°Ý6°»ë©…†
(犘<0.05)sé
E-É-Gm,Uh29—ÞLj[\Gmh°;
L50mg·L-1MnÓùœE+É-Gm°»ë
…†
(犘<0.05)séE-É-Gm,èg3Lã,
801
!1# u¹Š?:ø、QƒW29—ÞöcDÉ-Gmh°TUV
Mn629—ÞDÉ-GmT°Ý6°»ë ÞJæöcd。
`1 c~x€ 犕狀noPÀÁŒe2‘’
Table1 Seedsgerminationof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊underthedifferentconcentrationofMn
Mn
/mg·L-1
°Ý
Germinationrate/%
°»ë
Germinationindex
E+ E- E+ E-
0 96.67±3.33A 84.44±3.85a 21.58±0.08A 18.83±0.73a
50 88.89±1.92AB 86.67±5.77a 19.92±0.30B 17.94±0.49a
100 86.67±8.82AB 85.33±3.33a 19.61±1.04B 18.94±0.97a
200 85.56±5.09B 85.11±5.09a 18.42±0.46C 18.05±0.93a
  ü:g&@j6@YU;Çg˜ÜÞJr(E+)%غ…†(犘<0.05),uU;Çg˜6ÜÞJr(E-)%غ…†(犘<0.05),g˜E
+6E-ã%غ…†(犘<0.05),œ@
Note:Differentcapitalletterwithinthesamecolumnmeanssignificantdifferencewheninfectedendophyteatthe0.05level;differentlow
ercaseletterswithinthesamecolumnmeanssignificantdifferenceoffreeendophytetreatmentatthe0.05level. meanssignificantdifference
atthe0.05levelbetweenE+andE-.Thesameasbelow
2.2 SA$E+wE-ÀÁŒe‘’2|u
èg2Lã,ÀìSAÓù×ATíî,E+
É-Gm°Ý6°»ë©„jês_
€TóI

@:
,E-É-Gm°Ý6°
»ë҄jês_€TŒ:<ðóI

L0,
5,10,15mg·L-1SA Óù×Aœ,E+É-
Gm°Ý6°»ë©Øº6…†

L10
mg·L-1SA Óù×Aœ,E-É-Gm°
݅†
(犘<0.05)séSADÒ,‘/Óù×A
œØº©6…†

èg4Lã,29—Þ6SA
DÉ-T°Ý©J[\cd

Iñ™îJ
æöcd

`2 c~x€犛犃noPÀÁŒe2‘’
Table2 Seedsgerminationof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊underdifferentconcentrationofSA
SA
/mg·L-1
°Ý
Germinationrate/%
°»ë
Germinationindex
E+ E- E+ E-
0 92.22±1.92A 83.22±3.34b 19.03±0.05A 18.25±0.82ab
5 94.44±5.09A 88.90±1.92ab 19.08±0.96A 19.28±1.04a
10 94.44±5.09A 92.22±1.92a 19.86±0.59A 19.02±0.45a
15 88.89±5.09A 86.67±6.67ab 19.11±1.08A 16.88±0.35b
2.3 Mnno$ÀÁŒ“ì‡w“‡2|u
~ë11˜,Àì MnÓù×ATíî,E+
6E-É-}Ÿ©„h…œTóI。L Mn
n100,200mg·L-1Óùœ,E+É-}Ÿ…
†
(犘<0.05)œ,0mg·L-1ˆ¸,=¾œ
¡18%645%,L Mnn50,100,200mg·L-1
Óùœ
,E-É-}Ÿ…†(犘<0.05)œ,
0mg·L-1ˆ¸,=¾œ¡18%,34%653%;
E+É-ŸÀ Mn×ATíîRŒœ,L
Mnn100mg·L-1Óùœ…†(犘<0.05)€é


€¡11%,E-É-ŸÀ Mn×AT
íî҄RŒœóI

L Mnn200mg·L-1
Óùœ…†
(犘<0.05)€éDÒ,€¡12%。
èg2Lã,Mn629—ÞDÉ-GmTæ
}Ÿ6æŸTUVغ…†

òñ™ã%+L
æöcd

=1 犕狀no$ÀÁŒì‡w‡2|u
Fig.1 EffectsofMnonrootandshootlengthof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seedlings
901
! " # $ !24"
`3 犕狀wÞcbR5$ÀÁŒe‘’w“쓇2|u
Table3 EffectsofMnandendophyteon犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊germinationandrootandgerminallength
ÒØç”Source ¶èAdf 犉 …†Sig.
°Ý
Germinationrate
29—ÞEndophyte 1 0.07 0.79
Mn×A Mnconcentration 3 0.41 0.75
29—Þ×Mn×AEndophyte×Mnconcentration 3 4.49 0.01
°»ë
Germinationindex
29—ÞEndophyte 1 31.08 <0.01
Mn×A Mnconcentration 3 9.10 <0.01
29—Þ×Mn×AEndophyte×Mnconcentration 3 4.83 0.01
æ}Ÿ
Rootlength
29—ÞEndophyte 1 0.71 0.41
Mn×A Mnconcentration 3 43.87 <0.01
29—Þ×Mn×AEndophyte×Mnconcentration 3 1.64 0.22
æŸ
Germinallength
29—ÞEndophyte 1 14.04 <0.01
Mn×A Mnconcentration 3 4.06 0.03
29—Þ×Mn×AEndophyte×Mnconcentration 3 2.19 0.13
2.4 SAno$ÀÁŒ“ì‡w“‡2|u
~ë21˜,Àìe”SA×ATíî,E+É
-}Ÿ6Ÿ©„Œ:SêóI

Le”SA×
An15mg·L-12,E+É-}Ÿ6Ÿ©„…
†
(犘<0.05)séDÒ,©íî¡12%;E-É-
}Ÿ6Ÿ<ðóIRŒ

©ï…†Øº

-e”
SAÓù×An0,5,15mg·L-12,E+É-}
Ÿ…†
(犘<0.05)séE-É-}Ÿ。èg3L
ã
,SA629—ÞDÉ-æ}6æT9ŸÞ
J…†T[\cd

òñ™ã%ÞJæöcd

=2 ÃA犛犃$ÀÁŒ“ìw“‡2|u
Fig.2 EffectsofexogenousSAonrootandshootlengthof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seedlings
`4 犛犃wÞcbR5$ÀÁŒe‘’w“쓇2|u
Table4 EffectsofSAandendophyteon犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊germinationandlengthofrootandshoot
ÒØç”Source ¶èAdf 犉 …†Sig.
°Ý
Germinationrate
29—ÞEndophyte 1 7.99 0.01
SA×ASAconcentration 3 3.71 0.03
29—Þ×SA×AEndophyte×SAconcentration 3 0.44 0.73
°»ë
Germinationindex
29—ÞEndophyte 1 1.64 0.22
SA×ASAconcentration 3 2.74 0.08
29—Þ×SA×AEndophyte×SAconcentration 3 0.22 0.89
æ}Ÿ
Rootlength
29—ÞEndophyte 1 285.43 <0.01
SA×ASAconcentration 3 6.90 <0.01
29—Þ×SA×AEndophyte×SAconcentration 3 1.26 0.32
æŸ
Germinallength
29—ÞEndophyte 1 15.09 <0.01
SA×ASAconcentration 3 7.17 <0.01
29—Þ×SA×AEndophyte×SAconcentration 3 0.82 0.50
2.5 SAWÞcbR5$ MnnoPÀÁŒe
“ì“Â2|u
èë3Lã,Lˆ@×AT MnÓùœ,E
+É-}ŸÀìSA×ATíî„j€_
êsTóI

D҈¸

-SA×An5mg
·L-12,E+É-}Ÿ…†(犘<0.05)œ,
€25%,E-É-}ŸÀìSA×ATíî
Œ:Sê

غ6…†
;E+É-ŸÀìSA
011
!1# u¹Š?:ø、QƒW29—ÞöcDÉ-Gmh°TUV
×ATíî„RŒóI<ð

غ6…†
,E-
É-ŸÀìSA×ATíîŒ:Sê,غ
6…†

èg5Lã,L MnÓùœ,29—Þ
6SADÉ-æ}TæT9ŸÞJ…†(犘
<0.05)T[\cd,òñ™Dæ}T9ŸÞJ
æöcd

=3 ÃA犛犃$ 犕狀z{PÀÁŒ“ìw“‡2|u
Fig.3 EffectsofexogenousSAonrootandshootlengthofMntreated犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seedlings
`5 犛犃wÞcbR5$ 犕狀noPÀÁŒe“ì‡w“‡2|u
Table5 TheinteractionofSAandendophyteonrootandgerminallengthof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊byMnstressed
ÒØç”Source ¶èAdf 犉 …†Sig.
æ}Ÿ
Rootlength
29—ÞEndophyte 1 40.67 <0.01
SA×ASAconcentration 3 4.17 0.02
29—Þ×SA×AEndophyte×SAconcentration 3 6.36 <0.01
æŸ
Germinallength
29—ÞEndophyte 1 2.25 0.15
SA×ASAconcentration 3 1.59 0.23
29—Þ×SA×AEndophyte×SAconcentration 3 3.08 0.06
3 ˆ‰Wp‰
  »BCŒ“/0•¸¬ MnÓùœE+6E-
É-GmTh°xö

j|e”SAD MnÓù
œGmh°)TŒ”cd

ÆòXy¡ Mn62
9—Þ
、SA629—Þ| MnÓùœSA629—
ÞTæöcd

3.1 MnwSAno$E+wE-ÀÁŒe‘’
2|u
MnÚJK9Ÿ1ò3TEDüu,‘×AY
uÚg©cK¤`61FT‰5uãß
[37]。
}
Ï{L
[38]
³PBCLã

É-‡P9Ÿ13 Mn
TÝK×An0.01mmol·L-1,è.Lã,‘13
`ÿƒ

nÀìÃ)ðT6ÿ°±

ÙC6YŠ&
T•¸¬»`4Yé.×A
[39],
=`T Mn(D
JK+’9ùú5

)5^ÆòUVó¦Fo
Ø
[40],
DÉ-9K`TÖ¹6ÈÉT‡P9Ÿ
°JJ)cd
[38]。
/0gh

q-29—Þ§
›sËÌJKDEDKFTÏd:Ý

Ín[JK9Ÿ
[41],
q-29—Þ]Lj<=UVJK
ã%Tð0cd[\$)GmTh°

}Ÿ6
ɟ
[40,42]。
jSefˆQ

»/0°P

L Mn
DҜ
,E+É-Gm°Ý6°»ë©…†
séE-É-Gm,Uh29—ÞT+LDÉ
-GmTh°ÞJ[\cd

.Zhang[19]/0
ˆÙ

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