全 文 :!24" !1#
Vol.24 No.1
! " # $
ACTA AGRESTIA SINICA
2016$ 1%
Jan. 2016
犱狅犻:10.11733/j.issn.10070435.2016.01.015
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、
ó¿qWÞcbR5$ÀÁe2|u
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67 ÈY730020)
78
:
»/0<=íS,°BC
,
h"6@×AT MnÓùDÜ(E+)6Ü29Þ(E-)É-(犃犮犺
狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊)Gmh°T)cdj|eQW(salicylicacid,SA)D Mnú5Tcd。efgh,
¼î MnÓù¸,E+É-Gm°Ý6°»ë©
(犘<0.05)séE-É-Gm,Àì MnÓù×A
TíY
,E+É-GmTh°Ý©
(犘<0.05),nE-É-Gm°Ý6°»ëï
غ;Àì
MnÓù×ATíî,E+6E-É-}©h
TóI,nï
غ;LeSAÜÓù×A,
E+É-}©
(犘<0.05)séE-É-},L10mg·L-1MnÓùá&=¾¼î6@×ATeSA,
ef
eSAD MnÓùÞJcd。ÓS1ð,29Þ»BUVÉ-GmTh°,¸¬ú5DÉ
-Gmh°ÞJß©T)cd
,
neSALjK-_.ßú5。
9:;
:Mn;SA;É-;29Þ;Gmh°
<=>?@
:S452 ABCDE:A AFG@:10070435(2016)01010707
犈犳犳犲犮狋狊狅犳犕犪狀犵犪狀犲狊犲犪狀犱犛犃狅狀犌犲狉犿犻狀犪狋犻狅狀狅犳犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊
犛犲犲犱犆狅狀狋犪犻狀犻狀犵狅狉犉狉犲犲狅犳犈狀犱狅狆犺狔狋犲
WANZhiwen,WANGPing,ZHANGXingxu,LIChunjie
(KeyLaboratoryofGrasslandFarmingSystems,ColegeofPastoralAgricultureScienceandTechnology,
LanzhouUniversity,Lanzhou,GansuProvince730020,China)
犃犫狊狋狉犪犮狋:Variousconcentrationsofmanganese(Mn)andsalicylicacid (SA)affectedgerminationof
drunkenhorsegrass(犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊)seedsthatwereinfectedwith(E+)orfree(E-)ofa犈狆犻
犮犺犾狅犲/犖犲狅狋狔狆犺狅犱犻狌犿endophyte.TheresultsshowedthatE+specimensshowedasignificantly(犘<0.05)
highergerminationrateandindexthanthatofunderMnfreetreatment.ThegerminationrateofE+speci
mensshowedattendofdecreasingongerminationratewiththeincreasingofMnconcentration.Therewere
significantdifferencesexistedbetweentreatments(犘<0.05);butthegerminationrateandindexofE-
specimenshadnosignificantdifferences.E+specimensshowedasignificantly(犘<0.05)higherroot
lengthwhenvariousconcentrationsofexogenousSAwereadded.TheresultsshowedthatexogenousSA
couldrelieftheeffectsofMnonseedgermination.
犓犲狔狑狅狉犱狊:Mn;SA;犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊;Endophyte;Germination
HIJK
:20141224;LMJK:20151013
NOPQ
:
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”973”´ µ(2014CB138702);¬¶·*+®¸´µ(31372366);öJúÁÂSÊ°±²³´µ(IRT13019)Å
5RST
:
u¹
(1992),È,67áQÉ,Ë/09,/0Òcnq-29Þt9N,Email:wanzhw14@lzu.edu.cn;<Ôc
Authorforcorrespondence,Email:chunjie@lzu.edu.cn
q-29Þ(Fungalendophyte)Úßà§B
Lq-&A=àú$Yú=9ý¹#
,
I6ËÌ
JK
Geú;TÞ
[1]。
µvþÀLû
&%Fq-&°P¡犖犲狅狋狔狆犺狅犱犻狌犿 ¬|J1
,犈狆犻犮犺犾狅ё¬T29Þ[2],.à29ÞT+L
kJKThi§¥J1í
,
Ì«5ns¡
ÌÌJKDÜG9K
[3]
6È9Kú5
[4]
Tc
,
~øö
[56]、
ø
[78]、
ø
[910]、
)
[1112]
6D¸¬ú5
[13]
Tc
。
É-
(犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊)Úq»*ÍÍ
-¬
(犃犮犺狀犪狋犺犲狉狌犿)$9-»JK,ÚÕ:Ò
á·-.Ì«T]Ú-ãß
,
LÂÜ
,
234
、
ª
、
、
67?
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)
©J=>
[14]。1994$V
+Bruehl?[15]LÔ¶ÕÂÜTÉ-G
m&°P¡29Þ
。2000$Z¹F6eö|[16]
L67É-2°P¡29Þ 犖犲狅狋狔狆犺狅犱犻
! " # $ !24"
um,2004$eö|?[17]L67É-2°P¡
29ÞÂG犖.犵犪狀狊狌犲狀狊犲,}ÏÝÂwÞý
ïâ£
,
PÂýïn犈狆犻犮犺犾狅犲犵犪狀狊狌犲狀狊犻狊[18]。2
9Þs¡É-Thi§¥
[19],
Ls×AT
¸¬ú5É-TcJß©Ts
[20]。
ÀìÃ)T°±
、
ETi>
[21]、
(Î
[22]
6ð
[23]
TY`d
,
¸¬´µ
[24]
òô
,
n-
·Ó"TßYHlþ
[25]。
jÜGïÂiOT
¸¬ô´µ¡ÙC6QF
;
LJK&Y`Ö¹T
¸¬67ôUV¡JKPT9°J69ù
9ðoØ
,
nò]Lj<=&KàD!K6Éà²
³+ß©ÄAT°±
[26]。
¸¬ø
(Mn)ÚJK
91ò3T?`üu
,
JKGÂtoØ=
Ä
,
Ì«cncd&øÁð½T5m
[27]。
K
`T MnL[\JrP9Æíî¤`,I-×
AQ=ß©<=
,
ÿDJK¤9Ú±cd
[2829]。
Ï
i½
,
QW
(salicylicacid,SA)LJKøHlú
5
[3031](
~&
、
p
、
)
、
、
¸¬?
)
6±
[32]
Ò
ÓÞJh
T:f
,
Lj¸¬TÚ±
[33]
6)
ú5DGmh°|ÈÉT)cd
[34],
íîJrT
¼69K`
[35]。
Gmh°2#T9ö"#UVJKT9
°J6¤`
[36],
5.
,
»BC/0L¸¬ Mn
ÓùÍÖÜ6ÜÞÉ-GmTh°xö
,
W
XSAD¸¬ú5Tcd,n\ß/0É
-D¸¬ú5Tø
、
9ù9ð|=mÁù0
©¡ß©®¯
。
1 UVWXY
1.1 jkUV
É-Gmé2011$Ô¶67á¯0Ït(E
102°52',N37°12)ÆbGéÈYY+Ð&,
2012$10%_TGm5℃Ð+é()ú?-
--GmF`|Ñ×C&!
(
ÈY
)
GmhË
,
2013$3%ÜÞ(E+)66ÜÞ(E-)É-
u
,2014$7%Ð_TÉ-Gmdé»B
C
。
1dBÜ MnCl2·4H2O、QW (salicylic
acid,SA)©j=Úñâ¼î;QnA5mQ。
1.2 jkXY
1.2.1 Mnp[ESAE+pE-|e>34}
~E_ý Mn2+Óùá×A=¾n0,50,100,200
mg·L-1,SA Ó ù á × A = ¾ n 0,5,10,15
mg·L-1;j¼î?`A5mQnDÒ。fgÓ
²³
,
h]W1òkAt`TGm
,
d5%TX
WâÛẻ10min,jd¶çQ ¼ë,Ud
°ôQ ¼3,dìíÐQ^。f"Ân9
cmTD¹,¹2jYìín°,,Ð`T
¸¬Ûáî7mLÂéD¹&。¿¹©Q>
30]É-Gm,¿MÓù3M*,MNGm
Th°ë
(
æ}Gm?
、
æAWdGmß
,
34nGmþÀ°
)。GZ025Fà¶!Ò
Dà&ÛAn
(20±1)℃,Ò2%n16D/8N。
°#%¿r24hMNß,!10d^²°Ý,
Ʋ߰»ë
。
°Ý
(%)=BGmT°ë/BGmë
×100。
°»ë
(犌犐)= ∑犌狋/犇狋,â&犌狋nL狋ò
T°ë
,犇狋n°áë。
1.2.2 [ESA MnWXdE+pE|e>3
4pE_ý GmÀ°ôQºG24h
_
,
bGé¼J2ìíTD¹,î10mg·L-1
Mn2+Óùá,LÓùá&¼î6@×A@ATSA,
SA×A=¾n0,5,10,15mg·L-1,>ÂéÎÛà
228±1℃Íh°,h°7d_^²æ}6æ
。
1.2.3 Lê 1JëÏ©d Microsoft
Excel2003N,Æcë。ÔdSPSS16.0forwin
dows^²=ÕÖ\غ
=,dDun
canâ\¸t。
2 pqW>r
2.1 Mnno$E+wE-ÀÁe2|u
èg1Lã,Àì MnÓù×ATíî,E+É
-Gm°Ý6°»ë©h
TóI
,
nE-É-Gm°Ý6°»ëï
غ。
-MnÓù×An200mg·L-12,E+É-Gm
°Ý
(犘<0.05)é MnDÒ,¡×
12%;50,100,200mg·L-1MnÓùE+É-
Gm°»ë©
(犘<0.05)é MnDÒ,=
¾¡×8%,9%615%;L MnDÒ,E+É
-Gm°Ý6°»ë©
(犘<0.05)sé
E-É-Gm,Uh29ÞLj[\Gmh°;
L50mg·L-1MnÓùE+É-Gm°»ë
(犘<0.05)séE-É-Gm,èg3Lã,
801
!1# u¹?:ø、QW29ÞöcDÉ-Gmh°TUV
Mn629ÞDÉ-GmT°Ý6°»ë ÞJæöcd。
`1 c~x 犕狀noPÀÁe2
Table1 Seedsgerminationof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊underthedifferentconcentrationofMn
Mn
/mg·L-1
°Ý
Germinationrate/%
°»ë
Germinationindex
E+ E- E+ E-
0 96.67±3.33A 84.44±3.85a 21.58±0.08A 18.83±0.73a
50 88.89±1.92AB 86.67±5.77a 19.92±0.30B 17.94±0.49a
100 86.67±8.82AB 85.33±3.33a 19.61±1.04B 18.94±0.97a
200 85.56±5.09B 85.11±5.09a 18.42±0.46C 18.05±0.93a
ü:g&@j6@YU;ÇgÜÞJr(E+)%غ
(犘<0.05),uU;Çg6ÜÞJr(E-)%غ
(犘<0.05),gE
+6E-ã%غ
(犘<0.05),@
Note:Differentcapitalletterwithinthesamecolumnmeanssignificantdifferencewheninfectedendophyteatthe0.05level;differentlow
ercaseletterswithinthesamecolumnmeanssignificantdifferenceoffreeendophytetreatmentatthe0.05level. meanssignificantdifference
atthe0.05levelbetweenE+andE-.Thesameasbelow
2.2 SA$E+wE-ÀÁe2|u
èg2Lã,ÀìSAÓù×ATíî,E+
É-Gm°Ý6°»ë©jês_
TóI
,
@:
,E-É-Gm°Ý6°
»ëÒjês_T:<ðóI
。
L0,
5,10,15mg·L-1SA Óù×A,E+É-
Gm°Ý6°»ë©Øº6
;
L10
mg·L-1SA Óù×A,E-É-Gm°
Ý
(犘<0.05)séSADÒ,/Óù×A
غ©6
;
èg4Lã,29Þ6SA
DÉ-T°Ý©J[\cd
,
IñîJ
æöcd
。
`2 c~x犛犃noPÀÁe2
Table2 Seedsgerminationof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊underdifferentconcentrationofSA
SA
/mg·L-1
°Ý
Germinationrate/%
°»ë
Germinationindex
E+ E- E+ E-
0 92.22±1.92A 83.22±3.34b 19.03±0.05A 18.25±0.82ab
5 94.44±5.09A 88.90±1.92ab 19.08±0.96A 19.28±1.04a
10 94.44±5.09A 92.22±1.92a 19.86±0.59A 19.02±0.45a
15 88.89±5.09A 86.67±6.67ab 19.11±1.08A 16.88±0.35b
2.3 Mnno$ÀÁìwÂ2|u
~ë11,Àì MnÓù×ATíî,E+
6E-É-}©h
TóI。L Mn
n100,200mg·L-1Óù,E+É-}
(犘<0.05),0mg·L-1¸,=¾
¡18%645%,L Mnn50,100,200mg·L-1
Óù
,E-É-}
(犘<0.05),
0mg·L-1¸,=¾¡18%,34%653%;
E+É-À Mn×ATíîR,L
Mnn100mg·L-1Óù
(犘<0.05)é
DÒ
,
¡11%,E-É-À Mn×AT
íîÒRóI
,
L Mnn200mg·L-1
Óù
(犘<0.05)éDÒ,¡12%。
èg2Lã,Mn629ÞDÉ-GmTæ
}6æTUVغ
,
òñã%+L
æöcd
。
=1 犕狀no$ÀÁìwÂ2|u
Fig.1 EffectsofMnonrootandshootlengthof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seedlings
901
! " # $ !24"
`3 犕狀wÞcbR5$ÀÁewìÂ2|u
Table3 EffectsofMnandendophyteon犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊germinationandrootandgerminallength
ÒØçSource ¶èAdf 犉
Sig.
°Ý
Germinationrate
29ÞEndophyte 1 0.07 0.79
Mn×A Mnconcentration 3 0.41 0.75
29Þ×Mn×AEndophyte×Mnconcentration 3 4.49 0.01
°»ë
Germinationindex
29ÞEndophyte 1 31.08 <0.01
Mn×A Mnconcentration 3 9.10 <0.01
29Þ×Mn×AEndophyte×Mnconcentration 3 4.83 0.01
æ}
Rootlength
29ÞEndophyte 1 0.71 0.41
Mn×A Mnconcentration 3 43.87 <0.01
29Þ×Mn×AEndophyte×Mnconcentration 3 1.64 0.22
æ
Germinallength
29ÞEndophyte 1 14.04 <0.01
Mn×A Mnconcentration 3 4.06 0.03
29Þ×Mn×AEndophyte×Mnconcentration 3 2.19 0.13
2.4 SAno$ÀÁìwÂ2|u
~ë21,ÀìeSA×ATíî,E+É
-}6©:SêóI
。
LeSA×
An15mg·L-12,E+É-}6©
(犘<0.05)séDÒ,©íî¡12%;E-É-
}6<ðóIR
,
©ï
غ
。
-e
SAÓù×An0,5,15mg·L-12,E+É-}
(犘<0.05)séE-É-}。èg3L
ã
,SA629ÞDÉ-æ}6æT9Þ
J
T[\cd
,
òñã%ÞJæöcd
。
=2 ÃA犛犃$ÀÁìwÂ2|u
Fig.2 EffectsofexogenousSAonrootandshootlengthof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seedlings
`4 犛犃wÞcbR5$ÀÁewìÂ2|u
Table4 EffectsofSAandendophyteon犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊germinationandlengthofrootandshoot
ÒØçSource ¶èAdf 犉
Sig.
°Ý
Germinationrate
29ÞEndophyte 1 7.99 0.01
SA×ASAconcentration 3 3.71 0.03
29Þ×SA×AEndophyte×SAconcentration 3 0.44 0.73
°»ë
Germinationindex
29ÞEndophyte 1 1.64 0.22
SA×ASAconcentration 3 2.74 0.08
29Þ×SA×AEndophyte×SAconcentration 3 0.22 0.89
æ}
Rootlength
29ÞEndophyte 1 285.43 <0.01
SA×ASAconcentration 3 6.90 <0.01
29Þ×SA×AEndophyte×SAconcentration 3 1.26 0.32
æ
Germinallength
29ÞEndophyte 1 15.09 <0.01
SA×ASAconcentration 3 7.17 <0.01
29Þ×SA×AEndophyte×SAconcentration 3 0.82 0.50
2.5 SAWÞcbR5$ MnnoPÀÁe
ìÂ2|u
èë3Lã,L@×AT MnÓù,E
+É-}ÀìSA×ATíîj_
êsTóI
。
DÒ¸
,
-SA×An5mg
·L-12,E+É-}
(犘<0.05),
25%,E-É-}ÀìSA×ATíî
:Sê
,
غ6
;E+É-ÀìSA
011
!1# u¹?:ø、QW29ÞöcDÉ-Gmh°TUV
×ATíîRóI<ð
,
غ6
,E-
É-ÀìSA×ATíî:Sê,غ
6
。
èg5Lã,L MnÓù,29Þ
6SADÉ-æ}TæT9ÞJ
(犘
<0.05)T[\cd,òñDæ}T9ÞJ
æöcd
。
=3 ÃA犛犃$ 犕狀z{PÀÁìwÂ2|u
Fig.3 EffectsofexogenousSAonrootandshootlengthofMntreated犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seedlings
`5 犛犃wÞcbR5$ 犕狀noPÀÁeìwÂ2|u
Table5 TheinteractionofSAandendophyteonrootandgerminallengthof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊byMnstressed
ÒØçSource ¶èAdf 犉
Sig.
æ}
Rootlength
29ÞEndophyte 1 40.67 <0.01
SA×ASAconcentration 3 4.17 0.02
29Þ×SA×AEndophyte×SAconcentration 3 6.36 <0.01
æ
Germinallength
29ÞEndophyte 1 2.25 0.15
SA×ASAconcentration 3 1.59 0.23
29Þ×SA×AEndophyte×SAconcentration 3 3.08 0.06
3 Wp
»BC/0¸¬ MnÓùE+6E-
É-GmTh°xö
,
j|eSAD MnÓù
Gmh°)Tcd
,
ÆòXy¡ Mn62
9Þ
、SA629Þ| MnÓùSA629
ÞTæöcd
。
3.1 MnwSAno$E+wE-ÀÁe
2|u
MnÚJK91ò3TEDüu,×AY
uÚg©cK¤`61FT5uãß
[37]。
}
Ï{L
[38]
³PBCLã
,
É-P913 Mn
TÝK×An0.01mmol·L-1,è.Lã,13
`ÿ
,
nÀìÃ)ðT6ÿ°±
,
ÙC6Y&
T¸¬»`4Yé.×A
[39],
=`T Mn(D
JK+9ùú5
,
)5^ÆòUVó¦Fo
Ø
[40],
DÉ-9K`TÖ¹6ÈÉTP9
°JJ)cd
[38]。
/0gh
,
q-29Þ§
sËÌJKDEDKFTÏd:Ý
,
Ín[JK9
[41],
q-29Þ]Lj<=UVJK
ã%Tð0cd[\$)GmTh°
、
}6
É
[40,42]。
jSefQ
,
»/0°P
,
L Mn
DÒ
,E+É-Gm°Ý6°»ë©
séE-É-Gm,Uh29ÞT+LDÉ
-GmTh°ÞJ[\cd
,
.Zhang[19]/0
Ù
。
·n
,Hesse?[43]T/0gh,犖犲狅狋狔狆犺狅犱
犻狌犿¬T29ÞJs$9^-(犔狅犾犻狌犿
狆犲狉犲狀狀犲)h°|Gm¤`T§¥,IDÞr\
J2°P
,
.G§¥Æ6Úß"+L
,
@}®5F
T29ÞD^-Gmh°6¤`îJUV
。
.ßiyT+LL§BC&1dT29Þj
|ÌÌJKT®5F$9\FJ
。
Àì MnÓ
ù×ATíî
,E+É-Gm°Ý6°»ë
©h
TóI
,E-É-Gm°Ý6°
»ëÒóI
,
Iغ6
,
É-Gm
T°Ý6°»ëcNSE+séE-,D Mn
629Þ\=
,
ef
ñ+Læöc
d
。
UhL×A Mn(<50mg·L-1)Óù,2
9ÞLj MnDGmTú5,Is×A(>
100mg·L-1)T Mn67DÉ-Gm»BÞJ
)cd
,
]Lj\Gm2ú)29Þ@
}ÂtoØ
,
É-29Þt9NT¸
¬§¥
,
)ÁùJR\ß/0
。
111
! " # $ !24"
3.2 犕狀,犛犃wÞcbR5$ÀÁeì
Â2|u
/0gh
,
eQWD¸¬¦ú5JK
Gmh°6ÈÉT9ÞJß©T:
[44],
SAÓù§CdDGmh°6ÈÉ9T-±
JrN2CAT,APX,SOD6POD?[45]øÁð
½É<ðJ
,SA]Ljs
WT»`
iÓ$)ú5DJKTÚ±
[46],
;Ÿ¬6¥
DH.T1B
[47]。
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