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作 者 ：李琰;冯俊涛;王永宏;崔欢;张兴

期 刊 ：武汉植物学研究 2010年 28卷 2期 页码：224-228

关键词：雷公藤；愈伤组织；组织培养；抗褐变剂；次生代谢产物；

Keywords:Tripterygium wilfordii Hook f., Callus, Tissue culture, Browning inhibitors, Secondary metabolites,

摘 要 ：

以雷公藤(Tripterygium wilfordii Hook f.)根愈伤组织为材料,以NT为基本培养基,添加不同浓度硫代硫酸钠、抗坏血酸、柠檬酸、硝酸银、聚乙烯吡咯烷酮以及活性炭,探讨抗褐变剂对雷公藤愈伤组织生长、抗褐化和雷公藤内酯醇及总生物碱含量的影响.结果表明:除较低浓度柠檬酸以外,其他抗褐变剂均不同程度抑制雷公藤愈伤组织的生长.培养基中添加不同浓度硫代硫酸钠、抗坏血酸、柠檬酸、聚乙烯吡咯烷酮以及活性炭,雷公藤愈伤组织褐化程度不但没有得到控制,反而使褐化加重.但所有处理中,愈伤组织中内酯醇含量明显升高,其中加入 50 mg/L 柠檬酸处理的内酯醇含量为对照的2.4倍.培养基中加入10～50 mg/L硝酸银不仅能抑制雷公藤愈伤组织褐变,内酯醇的含量也随着硝酸银浓度的增加而增加.供试抗褐变剂中除较低浓度硫代硫酸钠以外,其他处理对雷公藤总生物碱的合成起抑制作用.

Abstract:To study the effects of browning inhibitors on callus growth and secondary metabolites content,the callus of Tripterygium wilfordii root and the NT were used as basic medium,supplemented with different concentrations of Na2S2O3,Vc,citric acid,AgNO3,PV,and active carbon.The results showed that callus growth in T.wilfordii was inhibited in all treatments,in addition to the citric acid with a lower concentration.Although the browning could not be controlled when the medium was supplemented with Na2S2O3,Vc,Citric acid，AgNO3，PV,and active carbon,the triptolide content increased significantly in all treatments,improving up to 2.4 times with the addition of 50 mg/L citric acid.However,the addition of 10-50 mg/LAgNO3 in the medium,inhibited callus browning and increased the triptolide content with the increase in AgNO3 concentration.Alkaloids content were inhibited by using other browning inhibitors,in addition to the Na2S2O3 with a lower concentration.

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