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作 者 ：张智奇;钟维瑾;唐克轩;周音;祝明福

期 刊 ：作物学报 1994年 20卷 05期 页码：578-581

关键词：异源三倍体；悬浮培养；琼脂糖包埋；植株再生；

Keywords:Allotriploid, Suspention, Agarose embedding, Plant regeneration,

摘 要 ：本试验利用二倍体栽培稻（Oryza stativa L.）品种8204，染色体组型为AA，2n=24作为母本，四倍体小粒野生稻（Oryza minuta）染色体组型为BBCC，2n=4x=48作父本杂交，获得异源三倍体杂交种，染色体组型为ABC。F1根尖检查，染色体数目为2n=3x=36。用该异源三倍体杂交种的幼穗诱导愈伤组织，经长期细代培养进行原生质体游离，获

Abstract:The experimental material was an allotriploid (ABC 2n=3x=36), the hybrid F1 of the cross O. sativa (AA 2n=24) × O. minuta (BBCC 2n=4x=48). Calli derived from young panicals of hybrid F1 through long-term subculture were used for protoplast isolation and plantlets were regenerated. The results indic

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