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作 者 ：李茂寅，赵德修，邢建民,赵丽丽

期 刊 ：云南植物研究 2000年 22卷 01期 页码：1-3

关键词：水母雪莲；愈伤组织培养；黄酮类化合物；

Keywords:Saussurea medusa, Callus Culture, Flavonids,

摘 要 ：8种基本培养基对水母雪莲(Saussurea medusa Maxim)愈伤组织生长和黄酮类形成影响不同，MS培养基较有利于愈伤组织生长和黄酮类形成。碳源、氮源、植物激素对愈伤组织生长和黄酮类形成影响较为显著。从MS培养基修饰得到的M-13培养基培养的愈伤组织生长量和黄酮类产量比原培养基分别提高33%和82%，达到21.00gDW/L和1.89g/L。通过TLC和HPLC初步分析和鉴定，证明M-13培养基上培养的愈伤组织能够形成2种有效黄酮类成分金合欢素(jaceosidin)和高车前(hispidulin)，其含量分别占黄酮类的22%～24%和0.15%～0.17%，这种百分率与MS培养基培养下的2种黄酮没有明显差异。

Abstract:Effects of eight basic media on callus grwth and flavoniods production of Saussurea medusa were different. MS medium was rether suitable for callus growth and flavooids production. Carbon source,nitrogen source and plant hormone had the obvious effects on the callus growth and flavonoids production. The maxiimum callus yield and flavonoids prodcution in the M-13 medium modified from MS medium reached 21.00g DW/L and 1.89 g/L, and increased 33% and 82% than those in MS medium respectively. The results of Preliminary identification by TLC and HPLC methods demonstrated that the callus cultured on both M-13 medium and MS medium had the cpability to synthesize jaceosidin and hispidulin. The percentages of jaceosidin and hispidulin in the flavonoids were 22%-24% and 0.15%-0.17% respectively,but they were not very different between the callus cultured on MS medium and M-13 medium.

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