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HPLC specific chromatograms of Xingnaojing injection

醒脑静注射液HPLC特征图谱研究



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HPLCspecificchromatogramsofXingnaojinginjection
YANGLixin1,2,FENGWeihong1,2,XIABohou3,LINLimei3,LIUWeiwei4,MIAOWanqing5,
GANGuofeng4,ZOUJigao4,LIChun1,2
(1.InstituteofChineseMateriaMedica,ChinaAcademyofChineseMedicalSciences,Beijing100700,China;
2.NationalEngineeringLaboratoryforQualityControlTechnologyofChineseHerbalMedicines,Beijing100700,China;
3.HunanUniversityofChineseMedicine,Changsha410208,China;
4.WuxiJiminkexinShanhePharmaceuticalCo.,Ltd.,Wuxi214028,China;
5.QingxuCountyPeople′sHospitalofShanxiProvince,Taiyuan030400,China)
[Abstract] ToestablishandanalyzetheHPLCspecificchromatogramsofXingnaojinginjectionmanufacturedbydiferentfactories
TheseparationwasperformedonaThermoBDSHypersilC18column(46mm×250mm,5μm),withthemobilephaseconsistingof
acetonitrile002% formicacidaqueoussolutionforgradientelutionTheflowratewas10mL·min-1,andthecolumntemperature
was35℃Thedetectionwavelengthwassetat254nm,andthesamplesizewas20μLElevenchromatographicpeakswereidentified
ascharacteristicpeaksofHPLCspecificchromatogramsofXingnaojinginjection,afteranalyzing29batchesofXingnaojinginjection
samplesComparedwiththereferencesubstances,sevenofthemwereidentifiedaseucarvone,camphor,curcumenone,curcumenol,
curdione,curzerenoneandgermacrone,respectivelyHPLCspecificchromatogramsofXingnaojinginjectionmanufacturedbythreefac
toriescouldbeeasilyclassifiedintothreecategoriesafterinvestigationwithcomputeraidedsimilarityevaluationsystemcombinedwith
principalcomponentanalysisTheestablishedHPLCspecificchromatogramsprovideabasisforscientificevaluationandefectivecon
·0461·
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trolofthequalityofXingnaojinginjection
[Keywords] Xingnaojinginjection;specificchromatograms;principalcomponentanalysis(PCA);highperformanceliquidchro
matography(HPLC)
doi:10.4268/cjcmm20160912
  
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S1 10 120107 S16 10 150711
S2 10 110602 S17 10 150704
S3 10 100914 S18 10 150705
S4 5 110725 S19 10 150706
S5 10 120316 S20 10 150709
S6 10 100716 S21 10 150710
S7 10 101317 S22 10 1412172
S8 10 120405 S23 10 1411062
S9 10 110310 S24 10 1501032
S10 2 110211 S25 10 140512
S11 5 120215 S26 10 140606
S12 5 111206 S27 10 140705
S13 10 140301 S28 10 141214
S14 10 110701 S29 10 141011
S15 10 150107
  
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