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期 刊 ：中国中药杂志 2008年 33卷 20期 页码：2390.

关键词：苦参素；感染性休克；细胞因子；NF-κB；

Keywords:oxymatrine, septic shock, cell factor, NF-κB,

摘 要 ：目的：观察苦参素(oxymatrine，OMT)对感染性休克大鼠肺组织NF-κB等细胞因子的影响。方法：采用大鼠盲肠节扎穿孔法(CLP)复制大鼠感染性休克模型，随机将56只大鼠分为假手术组、OMT对照组、模型(CLP)组、CLP+OMT高、中、低剂量组、阳性对照(CLP+地塞米松)组。采用免疫组化法检测肺组织NF-κB(p65)及IkB-α的活性；放射免疫分析法测定肺组织匀浆中肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)的含量改变。结果：OMT能显著抑制肺组织NF-κB(p65)及IkB-α的活性(P<0.05)，降低肺组织匀浆中TNF-α及IL-6的含量(P<0.05)，并提高动脉血PaO2，SaO2含量，降低PaCO2，HCO-3含量；降低肺组织W/D比值及肺系数(PWI)。结论：OMT能通过抑制诱导NF-κB的激酶(NF-κB-inducing Kinase，NIK)的活化从而抑制细菌、病毒等对NF-κB的激活作用，减少TNF-α，IL-6等促炎因子的表达，进而对感染性休克大鼠肺损伤性病变发挥治疗作用。

Abstract:Objective: To explore the effects of oxymatrine (OMT) on NF-κB and other cell factors in rat lung tissue with septic shock. Method: Fifty-six male SD rats were randomly divided into 7 groups: sham operation group, OMT control group, model (CLP) group, CLP+OMT high, middle, low-dose group, positive control group. Changes in NF-κB (p65) and IkB-α activity in the pulmonary tissue were determined by immunohistochemical method, tumour necrosis factor-a (TNF-α) and interleukin-6 (IL-6) levels in pulmonary tissue were determined by radioimmunoassay. Result: OMT could decrease significantly the NF-κB (p65) and IkB-α activity in the pulmonary tissue (P<0.05), TNF-α and IL-6 levels in pulmonary tissue homogenate decreased markedly (P<0.05). OMT could elevate the content of PaO2, SaO2, decrease the content of PaCO2, HCO-3 and decrease the ratio between wet weight of the lung and dry weight of the lung and the PWI. Conclusion: OMT can inhibit NF-κB-inducing kinase (NIK), NF-κB activity and reduce the expression of proinflammatory factor (TNF-α, IL-6) and antagonize the lung injury in a rat model of septic shock.

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