Abstract:Objective: To isolate and elucidate the constituents of herbs of Polygala hongkongensis. Method: The constituents were isolated and purify by chromatographic on silica gel, Sephadax LH-20 and semi-preparative HPLC. The structures were determined by NMR and MS spectral analysis. Result: Six compounds were identified as euxanthone (1), 1, 3, 6-trihydroxyxanthone (2), 1, 4, 7-trihydroxy-3-methoxy-xanthone (3), p-hydroxybenzoic acid (4), 3, 4-dihydroxybenzoic acid (5), and methy 1 2, 5-dihydroxybenzoate (6). Conclusion: The known compounds 1-6 were isolated from this plant for the first time.