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期 刊 ：中国中药杂志 2007年 32卷 17期 页码：1795.

关键词：黄连；生地黄；小檗碱；药代动力学；HPLC；

Keywords:Rhizoma Coptidis, Radix Rehmanniae, berberine, pharmacokinetics, HPLC,

摘 要 ：目的：研究黄连与生地黄不同比例配伍对小檗碱在大鼠体内药动学的影响,探寻二者最佳配伍比例。方法：给各组大鼠分别灌胃给予黄连∶生地黄为1∶0,1∶1,1∶4,1∶8水煎剂,采用高效液相色谱(HPLC)法测定大鼠体内小檗碱的血药浓度,血药浓度-时间数据经DAS 2.0药代动力学软件分析,比较各组大鼠体内小檗碱药代动力学参数。结果：小檗碱在大鼠体内的代谢过程符合一级二室模型,权重W=1/C²,方差分析结果表明,4组中相同剂量的黄连配伍不同比例的生地黄,小檗碱C_max,AUC_0～t,AUC_0～∞有显著差异(P<0.05),其中,黄连与生地黄１∶８组C_max,AUC_0～t,AUC_0～∞最大。结论：黄连配伍大剂量的生地黄,可显著提高黄连中有效成分小檗碱的生物利用度。

Abstract:Objective: To study the effect of Rhizoma Coptidis and Radix Rehmanniae with the different ratio on the pharmacokinetics of berberine in rats. Method: 24 rats were grouped to 4 groups randomly. Decoction, in which the proportion of Rhizoma Coptidis to Radix Rehmanniae is 1∶0, 1∶1, 1∶4, 1∶8 differently, was intragastrically given to the 4 groups. HPLC was used to determine concentration of berberine in serum. We adopted DAS 2.0 to analysis pharmacokinetic parameters of berberine. Result: The concentration-time curves was all fitted to two-compartment model with a weight of 1/C². Difference of 4groups in C_max, AUC_0～t, AUC_0～∞ is significant (P<0.05). Conclusion: Radix Rehmanniae of large dose can effectively enhance berberine’s bioavailability in rats.

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