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期 刊 ：中国中药杂志 2008年 33卷 10期 页码：1192.

关键词：桂皮酸；高效液相色谱；代谢；药代动力学；

Keywords:cinnamon acid, HPLC, metabolism, pharmacokinetics,

摘 要 ：目的:比较研究新西兰兔口服桂皮酸、肉桂、金匮肾气丸后血清中桂皮酸的药代动力学。方法:采用RP-HPLC法分别测定新西兰兔口服桂皮酸、肉桂、金匮肾气丸后桂皮酸的血药浓度。色谱条件为Symmetry C₁₈(3.9mm×150 mm,5μm)柱,甲醇-1%冰醋酸水溶液(45∶55)为流动相,流速0.8 mL.min^-1,柱温35℃,检测波长285nm。所得结果用3p87软件计算动力学参数。结果:桂皮酸的线性范围0.06~15μg.mL^-1(r=0.999 7),最低检测限为0.054μg.mL^-1,桂皮酸在兔体内的药动学过程均符合二室模型。结论:本方法灵敏、专一、精密,可准确测定桂皮酸在兔体内的血药浓度,通过3种情况的药代动力学参数,揭示了桂皮酸在兔体内的药代动力学规律。

Abstract:Objective:To study relatively pharmacological activities of cinnamon acid in blood serum of rabbit administered cinnamon acid,cinnamon and Jingui Shenqi pills.Method:RP-HPLC determine and analysis blood serum sample from rabbits administered cinnamon acid, cinnamon and Jingui Shenqi pills.Condition of colour spectrum was Symmetry C₁₈(3.9 mm ×150 mm,5 μm) chromato bar,mobile phase was methanol-1% glacial acetic acid water-solution(45∶55),flow rate was 0.8 mL·min^-1,temperature of bar was 35 ℃,detection wave length was 285 nm.The serum pharmacokinetic parameters were calculated with 3p87.Result:Linear range of cinnamon acid is from 0.06-15 μg·mL^-1(r=0.999 7),the lowest detectability is 0.054 μg·mL^-1.Pharmacokinetic process of cinnamon acid in rabbit could be all fitted to two-compartment model.Conclusion:Sensitive and exclusive HPLC that adopt can exactly detect serum concentration in rabbits administerd cinnamon acid.Pharmacokinetic parameters of three conditions can reveal pharmacokinetics regularity of cinnamon acid in rabbit.

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