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期 刊 ：中国中药杂志 2007年 32卷 05期 页码：414.

关键词：雷公藤甲素；甘草酸；胶原诱导性关节炎；

Keywords:glycyrrhizin, triptolide, collagen induced arthritis,

摘 要 ：目的：观察雷公藤甲素与甘草酸配伍对CIA模型大鼠关节肿胀和血清TNF-α,IL-10水平的影响。方法：使用Ⅱ型胶原诱导出成功的关节炎模型大鼠60只,并随机分为模型组、雷公藤甲素组、甘草酸组、雷草1组、雷草2组和雷草3组,另设空白组。以关节炎指数和关节肿胀度作为疗效评判标准,并采用ELISA方法检测大鼠血清中抗Ⅱ型胶原抗体,TNF-α和IL-10的水平。结果：经雷公藤甲素配伍甘草酸治疗后,模型大鼠的关节炎指数和关节肿胀度均显著下降(P＜0.01),血清中抗Ⅱ型胶原抗体和TNF-α水平显著下降,而IL-10水平显著升高(P＜0.05,P＜0.01)。结论：甘草酸配伍雷公藤甲素治疗CIA,可以在保持疗效的前提下减少雷公藤甲素的用量。

Abstract:Objective: To observe the effects of combination therapy with glycyrrhizin (GL) and triptolide (TP) on collagen-induced arthritis (CIA) rats. Method: Sixty male SD rats were randomly divided into 6 groups: the model group, the TP group, the GL group, and combination 1, 2, 3 groups. The models were induced by collagen type Ⅱ. The arthritis index (AI) and the edema rate were detected as curative effect, and the level of antibodies to collagen, TNF-α and IL-10 were measured by ELISA. Result: The combination therapy with GL and TP significantly reduced the paw edema and arthritis index of CIA rats (P＜0.01), and the combination therapy can increase the level of IL-10, while decrease the level of TNF-α, and the level of antibodies to collagen decreased too (P＜0.05, P＜0.01). Conclusion： Combine 26.78 mg·kg^-1 GL with 13.40 μg·kg^-1 TP can significantly inhibited the CIA, and the effect equal to the dosage of 17.86 μg·kg^-1 TP. It supports the possible of GL in combination with TP to reduce the dose and side effects related to TP.

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