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期 刊 ：中国中药杂志 2008年 33卷 15期 页码：1881.

关键词：六味地黄丸；知柏地黄丸；桂附地黄丸；中药血清药物化学；

Keywords:Liuwei Dihuang Wan, Zhibai Dihuang Wan, Guifu Dihuang Wan, serum pharmacochmistry of traditional Chinese medicine,

摘 要 ：目的：首次进行六味地黄丸及其类方知柏地黄丸和桂附地黄丸的血中移行成分的比较研究，试图从体内直接作用物质的角度初步阐明地黄丸类方的配伍规律。方法：利用RP-HPLC建立六味地黄丸及其类方给药后的大鼠血清色谱指纹图，比较分析，确定各复方的血中移行成分；进而通过单味药样品的对照，鉴定出衍生方与主方的差异血中移行成分及生药来源。结果：六味地黄丸及类方出现了14个共有血中移行成分，另外，知柏地黄丸产生了5个血中移行成分，桂附地黄丸产生了2个特有血中移行成分。结论：血中移行成分的数目与状态从体内直接作用物质的层面上反映了六味地黄丸及其类方功效上的异同，提示从经典方剂及其类方的体内成分入手进行深入研究，将是阐明复方配伍规律的有效途径。

Abstract:Objactive: To compare the compoents absorbed into blood after oral administration of Liuwei Dihuang Wan, Zhibai Dihuang Wan and Guifu Dihuang Wan, and to elucidate the composing principle of the prescriptions related to Liuwei Dihuang Wan. Method: Using the method of RP-HPLC, the serum chromatograms of rats after oral administration of the three prescriptions were established. By comparing the chromatograms with that of the prescriptions and composing drugs, the origin and the difference of compounds absorbed into blood of Liuwei Dihuang Wan and the other two prescriptions were identified. Result: Fourteen compounds were detected in all of the serum after administration of three prescriptions reapectly. Additionally, five special compounds were detected in the serum of Zhibai Dihuang Wan, and two special compounds were detected in the serum of Guifu Dihuang Wan. Conclusion: The number of compounds absorbed into blood reflects the different effects of the three prescriptions, and it should be the prospective access to elucidate the composing principle of formula.

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