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期 刊 ：中国中药杂志 2005年 30卷 04期 页码：266.

关键词：雷公藤总萜片；雷公藤内酯醇；高效液相色谱；含量测定；

Keywords:tripterygium total terpenoids tablets, triptolide, HPLC, assay.,

摘 要 ：目的:建立雷公藤总萜片中雷公藤内酯醇含量的HPLC测定方法。方法:采用Lichrospher CN色谱柱,甲醇 水为流动相,梯度洗脱,检测波长 255 nm。结果:雷公藤内酯醇在 0 742~59 36μg·mL^-1线性关系良好,r=0.999 8,平均加样回收率为99.2%(n=5),RSD 1.7%。结论:方法简便快捷,无干扰,重复性好,可用于雷公藤总萜片的质量控制。

Abstract:Objective: To esdevelop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets. Method:A Lichrospher CN column was used with wethanol and water for gradient elution. The detection wavelength was set at 255 nm. Result: The linear relationship of the concentrations and peak areas was good in range of 0.742-59.4 μg·mL^-1 (r=0.999 8). The average recovery was 99.2%, RSD%=1.7%. Conclusion: The method is simple, rapid and accurate and can be used for quality control of the tablets.

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