Abstract:Objective:To study the chemical constituents of Bufo Siccus.Method:Based on silica column chromatography six compounds were obtained from the alcoholic extract of Bufo Siccus and identified by physico chemical and spectroscopic analyses.Result:The compounds were identified as cholesterol,β-sitosterol,resibufogenin,cinobufagin,bufalin and gamabufotalin.Conclusion:Studies on the chemical constituents of Bufo Siccus were reported for the first time.