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期 刊 ：中国中药杂志 1997年 22卷 03期 页码：159.

关键词：银杏叶；萜类内酯；原位荧光薄层色谱测定；

Keywords:Ginkgo biloba, terpene lactone, fluorometric TLC,

摘 要 ：建立了采用优化条件展开的薄层色谱进行热化学衍生荧光,原位薄层定量,同板同时测定银杏内酯A,B,C及白果内酯,最低检出限分别为0.12～0.35μg。灵敏度分别为0.25～0.5μg,较HPLC示差折光检测器提高约10倍,分析时效比提高6倍以上,精密度同板RSD2%～3%,异板RSD2.3%～3.7%,回收率95.3%～98.8%。

Abstract:A new TLC method for the assay of terpene lactones in Ginkgo biloba extract and its preparations has been established by means of optimized development condition and post chromatographic thermal fluorescence derivatization. Satisfactory results can be obtained through polynomial regression calibration. The data obtained by this method have been proved ten times higher in sensitivity than those obtained by HPLC refracto detector.

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