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期 刊 ：中国中药杂志 1999年 24卷 01期 页码：12.

关键词：RP-HPLC；人工冬虫夏草；腺苷；

Keywords:RP HPLC, fermented Cordyceps, adenosine,

摘 要 ：目的：采用反相高效液相色谱法测定４种人工冬虫夏草中腺甘的含量。方法：采用ＧｉｌｓｏｎＨＰＬＣ系统，ＤｕｐｏｎｔＣ１８色谱柱，甲醇 ０．０６ｍｏｌ／Ｌ磷酸二氢钾 四氢呋喃（１０∶１５０∶１．５）为流动相，检测波长为１６０ｎｍ，流速１ｍｌ／ｍｉｎ。结果：该法平均回收率９８．７％，相对标准偏差ＲＳＤ为１．１０％（ｎ＝５）。结论：该法快速简便、灵敏度高，同时介绍了超声提取法替代回流法的可行性。

Abstract:Objective:A method for determination of adenosine in four fermented Cordyceps products by RP HPLC is described.Method:C ₁₈ column was used with methanol 0.06mol/L potassium phosphate monobasic tetrahydrofuran(10∶150∶1.5)as mobile phase.The flow rate was 1ml/min and detected at 260nm.Results: The average recovery was 98.7% and relative standard deviation was 1.10%(n=5).Conclusion:This method is simple,rapid and sensitive.The paper introduces the feasibility of supersonic extraction method taking the place of reflux method.

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