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期 刊 ：中国中药杂志 1999年 24卷 01期 页码：44.

关键词：加哇；高效液相；甘草查耳酮甲；阿魏酸；异甘草素；川陈皮素；

Keywords:Pleurospermum hookeri var.thomsonii, HPLC, licochalcone A, ferulic acid, isoliquiritigenin, nobiletin.,

摘 要 ：目的：建立藏药加哇中甘草查耳酮甲、阿魏酸、异甘草素和川陈皮素的含量测定方法。方法：ＯＤＳ柱，流动相甲醇 １％醋酸（７３∶２７，２８∶７２，５９∶４１）和甲醇 水（６５∶３５），检测波长３４５，３２０，３４５，３３２ｎｍ。结果：甘草查耳酮甲、阿魏酸、异甘草素和川陈皮素含量分别为０．７２８，０．１２４，０．０８３０，０．０２３７ｍｇ／ｇ，平均回收率分别为９９．４５％，９８．１３％，９７．８１％，９６．５８％。结论：为藏药加哇的质量评价、质量控制和开发利用提供了依据。

Abstract:Objective:To establish a reversed phase HPLC method for separation and determination of licochalcone A,ferulic acid,isoliquiritigenin and nobiletin in Pleurospermum hookeri var.thomsonii.Method:Using ODS column with methanol water(containing 1% acetic acid)(73∶27,28∶72,59∶41) and methanol water(65∶35) as the mobile phase,the flow rate was 1ml/min,the column temperature was 35℃ and UV detection at 345,320,345,332nm,different extraction methods for the sample were compared.Result:The average recoveries were 99.45%,98.13%,97.81% and 96.58%,the relative standard deviations were 0.83%,0.99%,0.73% and 1.55%,and the contents were 0.728,0.124,0.0830 and 0.0237mg/g.Conclusion:This study provides a scientific basis for quality evaluation,quality control and resources development and utilization of P.hookeri var.thomsonii.

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