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作 者 ：于荣敏,叶斌,宋丽艳

期 刊 ：中草药 2007年

关键词：人工蛹虫草；高效液相色谱；指纹图谱；

Keywords:cultured Cordyceps militaris (L.) Link, HPLC, fingerprint,

摘 要 ：目的 建立人工蛹虫草药材的高效液相色谱指纹图谱。方法 利用Agilent ZOBAXSB-AqC18(250mm×4.0mm,5μm)色谱柱,甲醇5%～60%为流动相梯度洗脱,分析时间30min,检测波长260nm,进样量1μL,体积流量1.0mL/min;对样品超声提取条件以及流动相、检测波长等色谱条件进行了系统优化设计。结果 建立的指纹图谱中有11个共有峰,且方法学考察符合规定的标准,并以"中药色谱图分析和数据管理系统"软件对11个人工蛹虫草样品进行了相似度评价。结论 该法准确简便,可作为控制人工蛹虫草药材内在质量的有效手段。

Abstract:Objective To establish the HPLC fingerprint of cultured Cordyceps militaris. MethodsHPLC Method was used for the determination of C. militaris on Agilent ZOBAX SB-Aq C18 column (250 mm×4.0 mm, 5 μm), and measuring with methanol 5%—60% in 30 min as elution detective wavelength at 260 nm, injection sample of 1.0 μL and flow rate of 1.0 mL/min. The systematic optimized design of the chromatographic conditions, such as supersonic extraction, mobile phase, and detective wavelength as well was carried out. Results Fingerprint consisted of 11 common peaks and the result of methodology determination fitted to the related standards. The evaluation of cultured C. militaris by systematically comparing chromatograms with a professional analytical software recommended by National Institute for the Control of Pharmaceutical and Biological Products has been established. Conclusion The method is accurate, simple, and useful for the quality control of cultured C. militaris.

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