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Cloning and Expression Analysis of AP1-like Gene from Sedirea japonica Effects of curcumin on gut microbiota of interval sleep deprivation rats Real-time fluorescent quantitative PCR and its application in microbial ecology Optimization of real-time quantitative fluorescence PCR system in detecting cytochrome b gene of Prorocentrum donghaiense Lu. Estimating copy number of transgenic gene by real-time fluorescent quantitative PCR(TaqMan) Cloning and sequence analysis of PqGA20ox gene in Panax quinquefolium Effects of vegetable cultivation years on microbial biodiversity and abundance of nitrogen cycling in greenhouse soils. Molecular Cloning、Correlation Analysis and expression studies of 4CL Gene in Ziziphus jujuba Mill.‘Wuhexiaozao’ Development and Application of Real-time Fluorescent Quantitative PCR for Detection of Pseudomonas tolaasii Effects of Bagging Treatment on Sugar Metabolism and Related Gene Expression in‘Chili’Pear Fruits Effects of Bagging Treatment on Sugar Metabolism and Related Gene Expression in‘Chili’Pear Fruits Detection of Trichoderma asperellum colonization in soils by real-time fluorescent quantitative PCR