全 文 ：1 Introduction
The research on the polyphenols of areca is made as
there is an interest in its antioxidant activities and anti-
aging effects [1 -4 ]. Areca nut contains polysaccharides,
minerals, crude fiber, alkaloids and some phenolics [5].
The phenolics in areca nut are catechin dimers,
leucocyanidin dimers and leucopelargonidin [6 -7]. The
shoot of areca is edible, the seed is usually made into
areca quid and Areca catechu L. in many countries
have traditionally been used as herbal medicines.
Methods which described in many papers[8-10]to assay
the content and ingredients of polyphenols in some
samples based on liquid chromatographic technique,
whereas capillary electrophoresis （CE） can be used as an
alternative to High-Performance Liquid Chromatography
（HPLC） in the determination of polyphenols. The
method to determine phenolic compounds using CE
technique have been developed by several authors[11-12],
but none of them have determined polyphenols in areca
husk. In this work, areca husks were first analyzed for
their phenolic compounds. The main objective of this
work was determining and separating the ingredients of
polyphenols presented in areca husk in an optimum
condition by CE method and evaluating the polyphenol
antioxidant activities so as to provide a theoretical basis
for betel nut industry.
热带作物学报 2012， 33（4）： 717-725
Chinese Journal of Tropical Crops
收稿日期： 2011-08-26 修回日期： 2012-02-29
基金项目： 中国热带农业科学院环境与植物保护研究所业务费： “红棕象甲寄主选择的生理生化机制研究（No. 2011hzs1J013)”； 海南省重点
科技计划项目 “高产槟榔新品种的选育及栽培关键技术研究（No. ZDXM20100024)”。
作者简介： 李 专（1984 年—）， 男， 助理研究员。
Determination of Polyphenols Ingredients Present
in Areca Husk and Its Antioxidant Activity
LI Zhuan1, QI Jing2, ZHAO Songlin1
1 Coconut Research Institute, CATAS, Wenchang, Hainan 571339, China
2 College of Food Science, Hainan University, Haikou, Hainan 570228, China
Abstract This paper was developed for the simultaneous determination of betel nut shell in a variety of phenolic
compounds by high performance capillary electrophoresis method, analyzed the separation effect of different
concentrations and pH values of buffer on 10 analytes, finally determined the best electrolytic buffer was 0.1 mol/L,
pH 9.0 borate buffer, UV detection wavelength 280 nm, separation voltage 20 kV. The method is simple and fast,
within 20 min 10 kinds of phenolic materials can be completely separated, the detection limit is 0.5~4.5 mg/L. In
addition, this article further determines of the betel nut shell polyphenol antioxidant activity, by using three
evaluation of the antioxidant capacity of index, namely: the DPPH free radical scavenging ability, reduction ability
as well as ABTS free radical scavenging ability.
Key words Areca husk； Capillary electrophoresis； Polyphenols； Antioxidant activity
doi 10.3969/j.issn.1000-2561.2012.04.026
槟榔壳多酚组分及抗氧化活性的测定
李 专 1， 祁 静 2， 赵松林 1
1 中国热带农业科学院椰子研究所， 海南文昌 571339
2 海南大学食品学院， 海南海口 570228
摘 要 建立 测定槟榔壳中多种酚类物质的高效毛细管电泳方法， 分析不同浓度和不同 pH 值硼酸缓冲液对 10
种标准品的分离效果， 最后确定最佳缓冲液为 0.1 mol/L， pH 9.0 的硼酸缓冲液， 紫外检测波长为 280 nm， 分离电
压为 20 kV。 方法简便快速， 能在 20 min 之内将 10 种酚类物质完全分离开， 检测限为 0.5～4.5 mg/L。 此外， 进
一步测定了槟榔壳多酚的抗氧化活性， 选用 3 个评价抗氧化能力的指标， 即： 对 DPPH 自由基的清除能力、 对
还原能力以及对 ABTS 自由基的清除能力。
关键词 槟榔壳； 毛细管电泳； 多酚； 抗氧化活性
中图分类号 TQ461 文献标识码 A
第 33 卷热 带 作 物 学 报
2 Experiments
2.1 Chemicals
Standard solutions were prepared in 18mΩ ultra-
purified water from a Millipore Milli -Q integral water
purification system. A 0.15 mol/L H3BO3 solution at pH
9.0 was used as a buffer solution. The standard solutions of
the following polyphenols were prepared: （+）-catechin,
（-）-epicatechin, gallic acid, coumaric acid, chlorogenic
acid, caffeic acid, rutin, keampferol and naringenin from
Sigma Chemical Company. A stock standard solution of
1 000 mg/L was prepared in methanol and stored at 4℃
in dark conditions and working standard solutions were
prepared by diluting the stock standard solutions with
methanol. Solutions of 0.1 and 0.5 mol/L sodium
hydroxide were used for conditioning the capillary and
adjusting the pH of the buffer solution. The reagents
which were used for detecting the antioxidant activity of
areca husk extract as follow： 2, 2-diphenyl-1-picryl-
hydrazil（DPPH）, potassium ferricyanide, trichloroacetic
acid, ferric chloride, butylated hydroxytoluene （BHT）
and 3-ethylbenzothiazoline-6-sulfonic acid from Sigma
Chemical Co.
2.2 Apparatus
A P/ACE MDQ capillary electrophoresis unit
equipped with a UV detector and fused-silica capillary
（50 μm in diameter and 57 cm in length） from Beckman
Co. was used to separate and determine the ingredients
of polyphenols presented in areca husk extract. The
extraction of the polyphenols from areca husk was
carried out by using ultrasound -assisted method and
was performed in an ultrasonic cleaning bath. The
extract solution was filtered through a vacuum filter and
was c oncentrated by a rotavapor. Before the sample
was analyzed by CE unit， it was filtered through the
0.45 μm filter membrane. The determination of total
phenolic content and the antioxidant activity of areca
husk extract was carried out by using an ultraviolet -
visible spectrophotometer （UV-1 200）.
2.3 Sample preparation
The areca fruits were bought locally from the
research site of Coconut Research Institute of Chinese
Academy of Tropical Agricultural Science. Firstly， the
fruits were cut into several pieces and were dried
through the drying cabinet at 50 ℃ . The extraction of
the polyphenols was carried out by using ultrasound-
assisted method with 41%（V/V） ethanol to increase the
solubility of the desired compounds and improve the
mass transformation. The ratio of material to liquid
was 1: 20 and the extraction was conducted at 54 ℃
for 38 min in ultrasonic cleaning bath and then cooled
down slowly at room temperature. The solvent of the
eluent was removed through the rotavapor at 40 ℃ after
the elute solution filtered by a vacuum filter. The
residue was dissolved again by methanol and
transferred into a 50 mL flask and this solution was
stocked as a crud sample. When detected the
ingredients of samples by CE unit, the crud sample must
be filtered with an organic filter membrane （0.45 μm）
and then injected into the sample bottle of CE unit, but
for the detection of antioxidant activity there was no
necessary to filter with organic filter membrane.
2.4 The condition of CE
The operating condition of CE unit as follow: the
running buffer was the solution of 0.1 mol/L H3BO3 and
pH 9.0（pH value was adjusted with sodium hydroxide）.
The applied voltage was 20 kV, the average current was
97.0 μA, the temperature was 20 ℃ and the selected
wavelength was 280 nm. Sample was injected by
hydrodynamic injection for 5 s. The separation was
carried out from the positive to the negative electrode.
In order to maintain the capillary under good working
conditions, its surface was regenerated once a day by
consecutive washing with methanol（5 min）, followed by
ultrapure water （5 min） and then prepared 0.5 mol/L
sodium hydroxide （5 min） freshly, 0.1 mol/L sodium
hydroxide（5 min） and finally fresh buffer （5 min）. In
order to optimize the migration time and the peak shape
reproducibility, the capillary was flushed between
analyses with 0.1 mol/L sodium hydroxide （3 min）,
ultrapure water （3 min）and fresh buffer（5 min）. When
used under the conditions mentioned above, the capillary
showed good performance for about three months
without losing its initial efficiency.
2.5 Determination of total phenolic content of
areca husk extract
The total phenolic content of areca husk extract
was determined with the Folin -Ciocalteu method [13].
Briefly, 0.1 mL methanol solution as a sample solution
at different concentration was mixed with 2.0 mL of
2% （W/V）sodium carbonate and reacted for 2 min,
718- -
第 4 期 李 专等： 槟榔壳多酚组分及抗氧化活性的测定
then added to 0.1 mL Folin -Ciocalteu reagent which
freshly prepared in our laboratory. Finally, the solution
was brought up to 10 mL by adding distilled water.
After 30 min of reaction at ambient temperature, the
absorbance was evaluated at 720 nm. Gallic acid was
used as a standard solution; the total phenolic content of
extract was expressed as a gallic acid equivalent value.
Data were reported as MEANS ±SD for at least three
replications.
2.6 Determination of DPPH radical scavenging
activity of areca husk extracts
The free radical scavenging activity of areca husk
extracts was measured by 2, 2 -diphenyl -1 -picryl -
hydrazil （DPPH） according to the method adopted by
Siddhuraju P[14], 0.1 mL methanol solution of sample at
different concentration was mixed with 1.4 mL of DPPH
solution（in 95% ethanol） and then added 95% ethanol
to 3 mL and incubated at room temperature for 30 min
in dark. DPPH solution alone prepared as the control.
Sample blank was prepared by replacing the DPPH
solution with 95% ethanol. The absorbance of the
mixture was measured at 517 nm after incubation and
the scavenging activity was calculated as follow:
Scavenging/%=[1－（Asample－Asample blank）/Acontrol]×100 （1）
Asample-The absorbance of the sample
Asample blank-The absorbance of the sample blank
Acontrol-The absorbance of the control
2.7 Reducing power
The reducing power was determined according to
the improved method adopted by Ylmazeram [15]. 0.2 mL
of the different concentration of areca husk extracts was
mixed with 1 mL of sodium phosphate buffer（0.2 mol/L,
pH6.6） and 1 mL of 1% potassium ferricyanide, then the
mixture was incubated at 50℃ for 20 min. After 1 mL of
10% trichloroacetic acid was added, the mixture was
centrifuged at 4 000 r/min for 10 min. The upper layer
（2mL）was mixed with 2 mL of distilled water and 0.4 mL
of 0.1% ferric chloride solution, and the absorbance was
read at 700 nm in a UV spectrophotometer. The higher
absorbance indicated a higher reducing power. BHT
（butylated hydroxytoluene） was used as comparison.
2.8 Antioxidant activity by the 2, 2′ -Azino -bis
（3-ethylbenzothiazoline-6-sulfonic acid） （ABTS）
radical cation assay
The total antioxidant activity of areca husk extracts
was measured by the ABTS+radical cation decolouriza-
tion assay[16]. ABTS was dissolved in water to a 7 mmol/L
concentration.ABTSradical cation（ABTS+）was produc-
ed by reacting ABTS stock solution with 2.45 mmol/L
potassium persulfate and allowing the mixture to remain
in the dark at room temperature for 12 -16 h. The
reaction of ABTS + with antioxidants was determined
spectrophotometrically at 734 nm.
Prior to assay, the ABTS+ solution was diluted with
ethanol （1 ∶ 89 V/V）to reach a final absorbance of the
control of (0.7±0.02) at 734 nm at 30 ℃. Stock solutions
of areca husk extracts were prepared in methanol. After
the addition of 3.0 mL of diluted ABTS+solution till up
to 0.5 mL of antioxidant compounds （ultimate concen-
tration 0.111~0.555 mg/mL）, the mixture was taken to
30 ℃ exactly for 30 min after the initial mixing.
Appropriate solvent blanks and ABTS+ solution alone as
the control were also run in each assay. Triplicate
determinations were made at each dilution of the
standard, and the percentage inhibition of the blank at
734 nm was calculated.
Scavenging/%=[1－（Asample－Asample blank）/Acontrol]×100 （2）
Asample-The absorbance of the sample
Asample blank-The absorbance of the sample blank
Acontrol-The absorbance of the control
3 Results and discussion
3. 1 Separation conditions of polyphenol compounds
The CE method was mainly designed for the
separation and quantification of the main polyphenols
presented inAreca catecgu L., such as （-）-epicatechin,
（+）-catechin, coumaric acid and chlorogenic acid, which
were separated and could be identified and quantified
using the appropriate standards. The following 10
analytes were chosen, such as （- ) -epicatechin, （+ ） -
catechin, keampferol, rutin, naringenin, ferulic acid，
chlorogenic acid, coumaric acid, caffeic acid and gallic
acid for optimization objective. The optimization of both
chemical variables and instrumental variables were
carried out.
The separation effect of different pH（in the range of
8.5 to 9.5） of H3BO3 solutions at a certain concentration
were analyzed [see Fig. 1 （A, B, C）], and different
concentration （in the range of 0.05 ~0.15 mol/L） of
solutions at a appropriate pH were also analyzed [see
719- -
第 33 卷热 带 作 物 学 报
1+2 5
4
6
7
8
9 10
0.1 mol/L H3BO30.04
0.03
0.02
0.01
0.00
AU UA 280 nm
pH8.5 0.04
0.03
0.02
0.01
0.00
AU
A
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
3
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
UA 280 nm 0.1 mol/L H3BO3 pH9.00.04
0.03
0.02
0.01
0.00
AU
0.04
0.03
0.02
0.01
0.00
AU
31 2
4 5
6
7
8
9 10
B
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
0.1mol/L H3BO3 pH9.5UA 280 nm
1 2
3
4
5
6
7
8
9
10
0.030
0.025
0.020
0.015
0.010
0.005
0.000
-0.005
AU
0.030
0.025
0.020
0.015
0.010
0.005
0.000
-0.005
AU
C
D0.5 mol/L H3BO3 pH9.0
UA 280 nm0.04
0.03
0.02
0.01
0.00
AU
0.04
0.03
0.02
0.01
0.00
AU
1
2 3
45
6
7
8
9
10
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
/min
/min
/min
/min
t
t
t
t
720- -
第 4 期 李 专等： 槟榔壳多酚组分及抗氧化活性的测定
Fig.1（D, E, F）]. Finally the separation effect of 0.1 mol/L
H3BO3 solutions at pH 9.0 were the best（see Fig.1）. The
total time taken for the whole analysis was less than
20 min and the wavelength is 280 nm.
Finally, the instrumental variables were optimized.
The voltage, temperature and wavelength were
considered. By increasing the voltage（up to 30 kV）and
the temperature, the time of migration was reduced, but
high voltage may lead to significant loss of resolution
and peak efficiencies, and high temperature may result
in poorer sensitivity. The value of voltage and
temperature finally chosen are 20 kV, 20 ℃ . A UV
detector was used to determine the maximum of
absorbance of these compounds. All of them presented a
maximum of absorbance at 280 nm.
3.2 The performance of CE method
The calibration graphs were produced from results
obtained by injecting standard solutions in the range
100 ~300 μg/mL. Each point of the calibration graph
corresponded to the mean value obtained from three
independent area measurements. The limit of detection
（LOD） was calculated from the blank value plus 3
times its standard deviation, whereas the limit of
quantification （LOQ）was calculated from the blank
value plus 10 times its standard deviation. The
corresponding regression equation and other
characteristic parameters for the determination of the
phenolic compounds are shown in Table 1.
Twenty replicate analysis were performed on the
standard solutions（100 μg/mL for each compound）, in
order to evaluate the precision of the method for every
compound to be determined. In all cases, the value of
the relative standard deviation for the absorbance was
less than 6%.
The standard deviations of residuals and curve -
fitting level were obtained by analysis of variance
during the validation of the calibration model.
3.3 Analysis of areca husk extract
In order to avoid some components that we do not
considered, the extraction of analytes was achieved by
using ethanol solution（41%）. Fig .2 shows the electro-
phorogram of the sample where the different peaks can
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
0.04
0.03
0.02
0.01
0.00
AU
UA 280 nm
0.04
0.03
0.02
0.01
0.00
AU
1
2 3
4 5
6
7
8
9 10
E0.1 mol/L H3BO3 pH 9.0
0.04
0.03
0.02
0.01
0.00
AU
0.04
0.03
0.02
0.01
0.00
AU
UA 280 nm 0.15 mol/L H3BO3 F
/min
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
1
2 3
4
5+6
7
8 9 10
（A， B， C） is resulted from that the the separation effect of buffer at 9.0 pH is the best； (D， E，F) demon-
strated that the the separation effect of 0.1 mol/L buffer is the best. Peaks of electropherogram of ten standard
phenolic compounds as follow, 1=(-)epicatechin; 2=(+)catechin； 3=naringenin； 4= rutin； 5=keampferol； 6=
ferulic acid； 7=chlorogenic acid； 8=coumaric acid； 9=caffeic acid；10=gallic acid.
Fig. 1 Electrophorogram of 10 analytes
pH 9.0
/mint
t
721- -
第 33 卷热 带 作 物 学 报
0.0050
0.0045
0.0040
0.0035
0.0030
0.0025
0.0020
0.0015
0.0010
0.0000
-0.0005
-0.0010
-0.0015
0.0050
0.0045
0.0040
0.0035
0.0030
0.0025
0.0020
0.0015
0.0010
0.0000
-0.0005
-0.0010
-0.0015
4 5 6 7 8 9 10 11 12 13 14 15 16
min
1
2
3
4
5
6
Peaks：1=（-）-epicatechin；2=（+）-catechin；3= keampferol； 4= naringenin；
5=ferulicacid；6=chlorogenicacid.
Fig. 2 Electrophorogram of the areca husk sample
AU AU
analytes y=ax+b r R2 RSD/％（AU） LOD/(μg/mL) LOQ/(μg/mL)
（-）epicatechin y=19 793 x -893.6 0.997 0.995 4 3.0 4.415 2 14.72
（+）catechin y=20 081 x +10 592 0.999 0.997 5 6.0 3.301 1 11.00
naringenin y=18 477 x +7 303 0.997 0.993 2 5.2 3.038 6 10.13
rutin y=40 238 x -7 575.9 0.995 0.990 1 2.3 1.773 5 5.91
keampferol y=94 464 x +69 596 0.999 0.999 5 4.1 2.188 4 7.29
ferulic acid y=81 491 x +61 595 0.997 0.994 9 2.5 0.587 5 1.96
chlorogenic acid y=17 957 x +11 186 0.997 0.993 3 3.2 2.733 6 9.11
coumaric acid y=57 524 x+5.933 3 0.994 0.988 0 2.2 1.219 7 4.07
caffeic acid y=83 988 x+44 708 0.994 0.988 9 1.4 1.180 3 3.93
gallic acid y=108 129 x+25 055 0.997 0.994 9 2.5 1.566 2 5.22
Table 1 Linear regression equation and limit of detection of 10 analytes
Note： a=intercept; b=slope; r=correlation coefficient; R2=curve-fitting level obtained by analysis of variance for the validation of the
model; RSD=relative standard deviation; LOD=limit of detection; LOQ=limit of quantification; LOD and LOQ in μg/mL. (Buffer: 0.1 mol/L
H3BO3 at pH 9.0 , 20 kV; 20 ℃; 5 s pressure injection;280 nm.)
be clearly identified. The migration time obtained for
the standard solutions and for the areca husk samples
were different due to matrix effects. In order to identify
these peaks the addition standard method was used.
Spiked areca husk sample were prepared in order
to evaluate the accuracy of the method. Excellent
recoveries were obtained（see Table 2）. In all cases, the
T -test was applied for the slopes of the calibration
graphs and showed no significative statistical
differences. Consequently there is no evidence of
systematic error affecting the determination of these
analytes in areca husk by the proposed method.
Recoveries and final concentrations found by using the
standard addition method are shown in Table 2. Some
polyphenolic compounds, such as gallic acid, caffeic
acid, coumaric acid and keampferol are not found in the
sample. In any case, the peaks corresponding to
keampferol and ferulic acid almost overlapped.
3.4 The total phenol content of areca husk extract
The total phenol content of areca husk extract was
expressed as the gallic acid equivalent value. The unit
of total phenol content was defined as the concentration
of gallic acid having the equivalent antioxidant activity
expressed as areca husk extract solution. The standard
curve of gallic acidis showed as Fig.3 According to the
standard curve the total phenol content of areca husk
extract was calculated 1.11 mg/mL.
3.5 DPPH radical scavenging activity
The f ree radical scavenging activity was
determined by a stable radical 2, 2-diphenyl-1-picryl-
hydrazil （DPPH）. The reduction capability of DPPH
induced by antioxidants was assayed by the decrease in
absorbance at 517 nm. Fig.4 shows the free radical
scavenging activity of the sample with BHT as control
standard. For areca husk extracts and BHT, the DPPH
radical scavenging activity increased with increasing
t/
722- -
第 4 期 李 专等： 槟榔壳多酚组分及抗氧化活性的测定
a,b,c,d,e: different letters indicate there is significant difference be-
tween each concentration group, the same letters between each concen-
tration indicate there is no significant difference. (the meaning of letters
in the following graphs is the same as this.
Fig. 4 DPPH radical scavenging activity
of areca husk extract and BHT
90
80
70
60
50
40
30
20
10
0


sample
BHT
ab
c
d
e
e
c b
a
10 20 30 40 50
concentration/％
sc
av
en
gi
ng
ac
tiv
ity
/％ d
0.040
0.035
0.030
0.025
0.020
0.150
0.010
0.005
0.000
-0.005
0 0.02 0.04 0.06 0.08 0.10 0.12
y=0.0334 3 x-0.000 4
R2=0.996
concentration/（mg/mL）
ab
so
rb
ab
ce
Fig. 3 Standard curve of gallic acid
analytes concentration added/（μg/mL） concentration measured/（μg/mL） recovery/％
(-) epicatechin
100 109.47 109.5
150 159.26 106.2
(+) -catechin
100 104.19 104.2
150 138.74 92.5
keampferol
100 98.26 98.3
150 147.22 98.1
rutin
100 101.77 101.8
150 151.3 101．0
naringenin
100 106.54 106.5
150 156.2 104.1
ferulic acid
100 111．0 111．0
150 143.43 95.6
chlorogenic acid
100 113.4 113.4
150 157.6 105.1
coumaric acid
100 110.62 110.6
150 152.9 101.9
caffeic acid
100 92.24 92.2
150 158.7 105.8
gallic acid
100 87．0 87．0
150 136．0 90.7
200 203．0 101.5
200 191.15 95.5
200 196.7 98.3
200 213.47 106.7
200 208.78 104.4
97.3194.6200
200 212.96 101．0
200 200.3 100．0
200 203.43 101.7
200 190.26 95.1
Table 2 The result of average recovery
723- -
第 33 卷热 带 作 物 学 报
concentration and there was significant difference
between each concentration although the DPPH radical
scavenging activities of areca husk extract were
weaker than those of BHT.
3.6 Reducing power
The activity of antioxidant has been reported
concomitant with the reducing power. Fig. 5 shows
the reducing power of areca husk extracts with
BHT as control. The higher absor bance indicates
stronger reducing power. The reducing power of the
areca husk extracts increased with increasing
concentration and there was significant difference
between each concentration. The areca husk extracts
performed a high reducing power although weaker than
that of BHT.
3.7 ABTS radical scavenging activity
The sample and BHT showed very high scavenging
activity towards ABTS radical in a dose dependent
manner. Even at a very low concentration of 10% ,
nearly 100% scavenging activity is observed and at the
concentration of 20% BHT shows 100% scavenging
activity. The areca husk extracts performed a weaker
scavenging activity than that of BHT and there is no
significant difference among concentrations of 30% ,
40% and 50%（see Fig. 6）.
4 Conclusions
CE is proved to be a suitable analytic separation
technique for the identification and quantification of
polyphenols in areca husk. The method developed
allows polyphenols to be determined at low levels with
detection limits between 0.5 ~4.5 mg/L. Moreover, it
should be noted that the specific advantage of this
method are based on the fact that pre-treatment of the
sample is required, apart from filtration and a suitable
dilution when it necessary. Linearity, recovery,
precision and sensitivity are highly satisfactory. At the
same time, through the experiment we also prove that
there exist high antioxidant activities in the areca husk
extracts because of its high total phenolic content.
References
[1] Wetwitayaklung P, Phaechamud T, Limmatvapirat C, et al. The
study of antioxidant capacity in various parts of Araca catechu L[J].
Naresuan University Journal. 2006, 14（1）: 1-14.
[2] Lei D, Chan C P, Wang Y J, et al. Antioxidative and antiplatelet
effects of aqueous inflorescence Piper betel extract [J]. Agriculture
and Food Chemistry Journal. 2003, 51（7）: 2 083-2 088.
[3] Wang C K, Lee W H. Separation, characteristics and biological
activities of phenolics in areca fruit [J]. Journal of Agriculture and
Food Chemistry. 1996, 44（8）: 2 014-2 019.
[4] Lee K K, Cho J J , Choi J D. Anti-elastase and anti-hyaluronidase of
phenolic substance from Areca catechu as a new anti-aging agent[J].
International Journal of Cosmetic Science, 2001,23（6）: 341-346.
[5] Mujumder A M, Kapadi A.H, Pendse G S. Chemistry and
pharmacology of betel nut Areca catechu Linn [J]. Jounal of Plant.
Crops 1979（7）: 69-92.
[6] Mathew A G, Parpia H A B, Govindarajan V S. The nature of
the complex proanthocyanidins [J]. Phytochemistry, 1969, 8 （8） :
1 543-1 547.
[7] Nonaka G, Hsu F, Nishioka I. Structures of dimeric, trimeric and
tetrameric procyanidins from Areca catechu L [J]. Journal of the
Chemical Society, Chemical Communications, 1981（15）: 781-783.
[8] Wu Q L, Yang Y H, Simon J E. Qualitative and Quantitative HPLC/MS
determinationofproanthocyanidins inArecaNut（Areca catechu）[J].
Chemistry & Biodiversity, 2007, 4（12）: 2 817-2 826.
[9] He H M, He J. Determining tannic acid content of areca flower by
reversed-phaseHPLC[J]. Chinese Journal of ModernAppliedPharm-
acy, 1996, 13（1）: 19-21.
[10] Alekseeva M A, Eller K I Arzamastsev A P. Determining polyphenolic
components of common hop by reversed-phase HPLC[J]. Pharmaceu-
tical Chemistry Journal, 2004, 38（12）: 39-41.
sc
av
en
gi
ng
ac
tiv
ity
/％
10 20 30 40 50
concentration/％
100.2
100.0
99.8
99.6
99.4
99.2
99.0
98.8


sample
BHT
b
b bbb
aaa
a
b
Fig. 6 ABTS radical scavenging activity
of areca husk extract and BHT


sample
BHT
e
e d
d c
c
b
b a
a
concentration/％
10 20 30 40 50
1.2
1.0
0.8
0.6
0.4
0.2
0.0
Ab
so
rb
an
ce
（ 7
00
nm
）
Fig. 5 reducing power of areca husk extract and
BHT
724- -
第 4 期 李 专等： 槟榔壳多酚组分及抗氧化活性的测定
[11] Lourdes A, María T T, Angel R, et al. Determination of trans -
resveratrol and other polyphenols in wines by a continuous flow
sample clean -up system followed by capillary electrophoresis
separation[J]. Analytica Chimica Acta, 1998, 359（1）: 27-38.
[12] Arce L, Ríos A, Valcárcel M. Determination of anti -carcinogenic
polyphenols present in green tea using capillary electrophoresis
coupled to a flow injection system[J]. Chromatography Journal, 1998,
827（1）: 113-120.
[13] Yuan V Y, Bone D E, Carrington F. Antioxidant of activity of dulse
（Palmaria palmate）extract evaluated in vitro [J]. Food Chemistry,
2005, 91（3）: 485-494.
[14] Siddhuraju P. Antioxidant activity of polyphenolic compounds
extracted from defatted raw and dry heated TamarIndus indica seed
coat[J]. LWT-Food Science and Technology, 2007, 40（6）: 982-990.
[15] Ylmazer M, Stevens J F, Buhler D R. In vitro glucuronidation of
xanthohumol, a favonoid in hop and beer, by rat and human
liver microsomes[J]. FEBS Letters, 2001, 491（3）: 252-256.
[16] Re R, Pellegrini N, Proteggente A, et al. Antioxidant activity
applying an improved ABTS radical cation decolorization
assay [J]. Free Radical Biology and Medicine, 1999, 26 （9/10）:
1 231-1 237.
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