Based on the biology peculiarity of Liriodendron L., the non-bagged pollination technique was used widely in cross breeding of the genus. Theoretical analysis and indirect detection indicated that the pollen contamination rate of this pollination technique was no higher than 1%. But there is no evidence from direct detection by now. The pollen contamination rate produced in this pollination technique, for the first time, was detected by means of RAPD markers in this study. The results revealed that all the 93 progenies detected, derived from the non-bagged pollination technique, were progenies of the purpose male tree of the cross. No pollen contamination was found. Because the frequency of the 4 paternal marker fragments selected for the detection were relatively low in the population, the reliability of the pollen contamination rate detected was very high (>99%). Therefore, the non-bagged pollination technique is viable in Liriodendron . The authors also considered that RAPD markers had higher potential of use in other fields of botanic study such as pollen competition, pollen flow, pollen contamination of forest seed orchard, etc.
用RAPD标记检测鹅掌楸属种间杂交的花粉污染
李周岐1,2* 王章荣**
（1. 西北农林科技大学林学院，陕西杨凌712100；2. 南京林业大学森林资源与环境学院，南京210037）
摘要：根据鹅掌楸属(Liriodendron L.)的生物学特点,实践上常采用不套袋杂交授粉技术,理论分析和间接检测结果证明这种杂交授粉方式的花粉污染率低于1％,但到目前为止仍缺乏直接的检测证据.本试验首次采用RAPD标记对鹅掌楸属种间不套袋杂交授粉的花粉污染率进行了检测,结果证明93个受检不套袋杂交授粉子代均来自目的父本,而非花粉污染所致.通过对所用4个父本标记谱带在试验群体中出现频率的测定和分析,证明这种检测结果的可靠性大于99％,从而认为对鹅掌楸属进行不套袋杂交授粉是可行的.并就这一方法在植物学研究领域的应用潜力进行了讨论.
关键词： RAPD；花粉污染；鹅掌楸属；杂交育种
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