RAPD (random amplified polymorphic DNA) markers were generated from filaments of 15 Porphyra lines representing four important groups, P. yezoensis, P. haitanensis, P. katadai var. hemiphylla and P. oligospermatangia. Among the total 69 fragments generated by 6 selected primers (among 50 primers), 67 appeared to be polymorphic (97.1%). Cluster analysis based on the RAPD results was performed. The 15 Porphyra lines were divided into 3 groups. This result was consistent with that from taxonomy analysis. A DNA fingerprinting based on 8 bands amplified with OPN-02 and OPJ-18 was constructed and might be used in Porphyra variety identification. Five specific RAPD fragments of 5 Porphyra lines were isolated and cloned into pGEM-T easy vector. These five RAPD fragments may be useful in germplasm identification and property protection of Porphyra.
RAPD 标记在紫菜遗传多样性检测和种质鉴定中的应用
贾建航 王萍 金德敏 曲雪萍 王倩 李传友 翁曼丽 王斌*
（中国科学院遗传研究所，北京100101）
摘要：用RAPD技术对 4类紫菜 (Porphyrayezoensis,P .haitanensis,P .katadaivar.hemiphylla和P .oligosper matangia)的 15个无性系丝状体进行了遗传多样性分析 ,从 5 0个OPERON引物中经过初筛 ,其中 6个引物可以扩增出稳定的可重复的图谱。这 6个引物共扩增出了 6 9条带 ,多态性比例达 97.1%。根据RAPD结果将这 15个无性系聚类为 3个群 ,此聚类结果与分类地位基本相符。利用来自引物OPJ_18和OPN_0 2扩增的 8条多态性条带构建了紫菜的DNA指纹图谱 ,该图谱可以方便地应用于紫菜无性系的种质鉴定。本研究还回收并克隆了 5个紫菜无性系的 5条特异片段 ,将用于无性系鉴定、种质资源保护和产权保护。
关键词： RAPD；遗传多样性；聚类分析；紫菜；种质鉴定
全 文 ：