Abstract:In our search for bioactive compounds from the whole plant of Picria felterrae Lour., three new phenylethanoid glycosides, picfeosides A–C (1–3), along with five known phenylethanoid glycosides, namely wiedemannioside (4), acteoside (5), acteoside isomer (6), cis-acteoside isomer (7), and cis-acteoside (8), were isolated using several chromatographic purification steps, including semipreparation HPLC on RP-18. The structures of the new compounds were elucidated on the basis of extensive spectroscopic analysis.