Abstract:There are six species of the genus Glycyrrhiza, growing in China. Along with G. uralensis, the species G. inflata is one of the widely distributed in Gansu province and Xinjang autonomous region. This species has been used in medical practice for a long time and recorded in the Chinese Pharmacopoeia of 1977 edition. The chemical constituents of G. inflata has not been reported in detail. This paper reports the isolation and identification of twelve compounds including eight flavonoids, three triterpenoids and a phytosterol from the roots of G. inflata gathered from Jinta county, Gansu province. On the basis of chemical and spectroscopic data, their structures were identified as licocha-lcone A and B, licoflavone, liquiritin, liquiritigenin, isoliquiritigenin, 4′,7-dihydrox-yflavone, ononin, glycyrrhizic acid, glycyrrhetinic acid, ll-deoxyglycyrrhetinic acid and β-sitosterol respectively. Licochalcone A was the major component among the flavonoids. It‘s 13C NMR data were recorded and their assignments were given for first time.The 4,7-dihydroxyflavone and ononin were isolated from the genus Glycyrrhiza for the first time.