Abstract:Three genotypes were selected from 54 genotypes of sunflower (Helianthus annuus L.) to test the ability of bud regeneration by tissue and suspension cultures. Suspension cells delivered of the callus were originated from the hypocotyl explants. The growth rate of cells was set up in MS with addition of BA (0.5 mg/l) and 2.4-D (0.001 mg/l). The buds were regenerated directly from the callus when 2,4-D was removed from the medium.