Abstract:With a CI-301PS portable photosynthesis system as a measuring device, a field study on the characteristics of transpiration and the responses of transpiration (E), stomatal resistance (R) and water use efficiency (WUE) to shade in Ginkgo biloba L. grown in Mt. Lushan was conducted. The results showed that with sufficient water in soil, the highest transpiration rate in a sunny summer day appeared in the afternoon. The WUE was maximum at about 8 a.m. and then decreased at noon remarkably. Different responses of E, R and WUE to shade were noticed which indicated that G. biloba was very suitable to the present climate. Moreover, the temperature of air, photosynthetic active radiation and R were the dominant factors affecting transpiration.