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作 者 ：邓文红,沈应柏,李镇宇,张淑静,刘雪萍

期 刊 ：生态学报 2008年 28卷 8期 页码：4023~4029

关键词：反相高效液相色谱；柱前衍生；游离氨基酸；γ-氨基丁酸；马尾松；

Keywords:reversed-phase high performance liquid chromatography, pre-column derivation, free amino acid, gamma-aminobutyric acid, Pinus massoniana,

摘 要 ：建立了自动在线柱前衍生反相高效液相色谱法同时测定γ-氨基丁酸（GABA）和17种游离氨基酸含量的方法。以邻苯二甲醛-9-芴基甲基氯甲酸酯（OPA-FMOC）为衍生试剂进行衍生，Agilent Hypersil AA-ODS-C18色谱柱分离，梯度洗脱，二极管阵列检测器检测，在19min内分离测定了马尾松苗木针叶中GABA 和17种游离氨基酸的含量。该方法测定氨基酸的回收率高于90.1%，精密度和重现性均较好（相对标准偏差为0.21%~2.81%），经测定，发现马尾松被马尾松毛虫取食后，所测18种氨基酸总量明显降低，从418.3μg·g-1降低到310.4μg·g-1鲜重。

Abstract:An automated pre-column derivation procedure has been developed for the determination of gamma-aminobutyric acid (GABA) and 17 free amino acids by means of reversed-phase high performance liquid chromatography. GABA and the 17 free amino acids in needles of Pinus massoniana seedlings were determined with a 0-phthaldialdelhyde-9-fluore-nylmethyl chloroformate pre-column derivation in 19 min. The amino acid derivatives were separated on an Agilent Hypersil AA-ODS-C18 column by a gradient elution method and identified with a DAD detector. The recovery of amino acids by this method was more than 90.1% with high precision and reproducibility (its relative standard deviation ranged from 0.21% to 281%). The total amount of amino acids in the needles of P. massoniana seedling decreased decidedly from 418.3 to 310.4 μg?g-1 FW after insect damage.

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