Abstract:Kinds of pigments in flowers and inheritance of flower color of Primula vulgaris were studied by analyzing absorption spectrum and thin-layer chromatography (TLC) of pigments and by crossing analysis of different flower colors in order to get hereditary rules for seed production. The results show that there could be kinds of anthocyanins in colony or single primrose that forms various red, pink and blue colors of flowers. Depth of yellow was mainly determined by content of carotinoid. The color of white is recessive. Yellow and pink are dominant and they also have quantitative inheritance characteristics. The flower color of yellow and pink are independent in heredity. Color of blue was determined by recessive multi-genes and also had quantitative characteristics.